Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 30% (w/v) PEG400, 0.1 M MES pH 6.5, 0.1 M magnesium chloride | Rhodococcus opacus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4,5,6-tetrachlorocatechol | competitive inhibitor | Rhodococcus opacus | |
3,5-dichlorocatechol | competitive inhibitor | Rhodococcus opacus | |
4,5-dichlorocatechol | competitive inhibitor | Rhodococcus opacus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
3-chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.0015 | - |
4-Chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.003 | - |
catechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.004 | - |
4-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.0045 | - |
pyrogallol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.0065 | - |
3-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.097 | - |
Hydroxyquinol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.31 | - |
protocatechuate | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.7 | - |
2,3-Dihydroxybenzoic acid | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe3+ | contains Fe3+ | Rhodococcus opacus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
SDS-PAGE | Rhodococcus opacus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus opacus | P95607 | - |
- |
Rhodococcus opacus 1CP | P95607 | - |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography, phenyl-Sepharose column chromatography, and Superdex 200 gel filtration | Rhodococcus opacus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:benzoate-grown cell | - |
Rhodococcus opacus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-dihydroxybenzoic acid + O2 | - |
Rhodococcus opacus | ? | - |
? | |
3,4,5,6-tetrachlorocatechol + O2 | - |
Rhodococcus opacus | ? | - |
? | |
3,5-dichlorocatechol + O2 | - |
Rhodococcus opacus | ? | - |
? | |
3-chlorocatechol + O2 | 1.7% activity compared to catechol | Rhodococcus opacus | 2-chloro-cis,cis-muconate | - |
? | |
3-chlorocatechol + O2 | 1.7% activity compared to catechol | Rhodococcus opacus 1CP | 2-chloro-cis,cis-muconate | - |
? | |
3-methylcatechol + O2 | 104% activity compared to catechol | Rhodococcus opacus | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
3-methylcatechol + O2 | 104% activity compared to catechol | Rhodococcus opacus 1CP | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
4,5-dichlorocatechol + O2 | - |
Rhodococcus opacus | ? | - |
? | |
4-chlorocatechol + O2 | 2.7% activity compared to catechol | Rhodococcus opacus | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-chlorocatechol + O2 | 2.7% activity compared to catechol | Rhodococcus opacus 1CP | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | 71.5% activity compared to catechol | Rhodococcus opacus | 4-methyl-cis,cis-muconate | - |
? | |
catechol + O2 | 100% activity | Rhodococcus opacus | cis,cis-muconate | - |
? | |
catechol + O2 | 100% activity | Rhodococcus opacus 1CP | cis,cis-muconate | - |
? | |
hydroxyquinol + O2 | i.e. 1,2,4-trihydroxy benzene | Rhodococcus opacus | ? | - |
? | |
protocatechuate + O2 | i.e. 3,4-dihydroxybenzoate | Rhodococcus opacus | ? | - |
? | |
pyrogallol + O2 | i.e.benzene-1,2,3-triol | Rhodococcus opacus | alpha-hydroxymuconic acid | - |
? | |
pyrogallol + O2 | i.e.benzene-1,2,3-triol | Rhodococcus opacus 1CP | alpha-hydroxymuconic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 33000, SDS-PAGE | Rhodococcus opacus |
Synonyms | Comment | Organism |
---|---|---|
1,2-CTD | - |
Rhodococcus opacus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.04 | - |
protocatechuate | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.18 | - |
3-chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.27 | - |
4-Chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.28 | - |
Hydroxyquinol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.28 | - |
2,3-Dihydroxybenzoic acid | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
2.41 | - |
pyrogallol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
7.32 | - |
4-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
10.23 | - |
catechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
10.62 | - |
3-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00002 | - |
4,5-dichlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.00009 | - |
3,5-dichlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.036 | - |
3,4,5,6-tetrachlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
protocatechuate | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
0.4 | - |
2,3-Dihydroxybenzoic acid | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
2.8 | - |
Hydroxyquinol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
182 | - |
4-Chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
255 | - |
3-chlorocatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
535 | - |
pyrogallol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
1633 | - |
3-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
1828 | - |
4-methylcatechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus | |
3410 | - |
catechol | in 50 mM Tris/HCl, pH 7.2, 25°C | Rhodococcus opacus |