Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AgNO3 | 0.1 mM, 10 min, complete inhibition | Geobacillus sp. | |
CuSO4 | 0.1 mM, 10 min, complete inhibition | Geobacillus sp. | |
EDTA | 1 mM, 10 min, 20% inhibition | Geobacillus sp. | |
FeSO4 | 0.1 mM, 10 min, 12% inhibition | Geobacillus sp. | |
H2O2 | 0.1 mM, 10 min, 98% inhibition | Geobacillus sp. | |
mercaptoethanol | 0.1 mM, 10 min, 98% inhibition | Geobacillus sp. | |
o-phenanthroline | 0.05 mM, 10 min, 3% inhibition | Geobacillus sp. | |
streptomycin sulfate | 5%, 10 min, 80% inhibition | Geobacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
catechol | - |
Geobacillus sp. | |
0.286 | - |
3-methylcatechol | - |
Geobacillus sp. | |
0.4 | - |
4-methylcatechol | - |
Geobacillus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
96000 | - |
SDS-PAGE | Geobacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus sp. | - |
- |
- |
Geobacillus sp. G27 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
about 18fold after ammonium sulfate precipitation, DEAE-celullose, Sephadex G-150 and hydroxylapatite chromatographie | Geobacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
7.42 | - |
- |
Geobacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | assay at 60°C, pH 7.0 | Geobacillus sp. | ? | - |
? | |
3-methylcatechol + O2 | assay at 60°C, pH 7.0 | Geobacillus sp. G27 | ? | - |
? | |
4-methylcatechol + O2 | assay at 60°C, pH 7.0 | Geobacillus sp. | ? | - |
? | |
4-methylcatechol + O2 | assay at 60°C, pH 7.0 | Geobacillus sp. G27 | ? | - |
? | |
catechol + O2 | intradiol, assay at 60°C, pH 7.0 | Geobacillus sp. | cis,cis-muconate | - |
? | |
catechol + O2 | intradiol, assay at 60°C, pH 7.0 | Geobacillus sp. G27 | cis,cis-muconate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
C1,2O | - |
Geobacillus sp. |
catechol 1,2-dioxygenase | - |
Geobacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Geobacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 90 | only 3% enzyme activity lost at 50-70°C, at 80°C 22% lose of 22% of enzyme activity, below 50°C and 70°C rapidly decreased enzyme activity | Geobacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | - |
2 month: 100% enzyme activity left | Geobacillus sp. |
60 | - |
half-life: 40 min | Geobacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Geobacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | 45% of specific activity in pH range from 8-10.5 | Geobacillus sp. |