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Literature summary for 1.13.11.1 extracted from

  • Cha, C.
    Catechol 1,2-dioxygenase from Rhodococcus rhodochrous N75 capable of metabolizing alkyl-substituted catechols (2006), J. Microbiol. Biotechnol., 16, 778-785.
No PubMed abstract available
Search for more literature for 1.13.11.1

Inhibitors

Inhibitors Comment Organism Structure
HgCl2 1 mM, 52% inhibition Rhodococcus rhodochrous
PCMB 0.1 mM, 16% inhibition Rhodococcus rhodochrous
Sodium dithionite 1 mM, 36% loss of activity Rhodococcus rhodochrous
Tiron 0.5 mM, 20% loss of activity Rhodococcus rhodochrous

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Rhodococcus rhodochrous

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
35000
-
 2 * 35000, SDS-PAGE Rhodococcus rhodochrous
71000
-
 gel filtration Rhodococcus rhodochrous

Organism

Organism UniProt Comment Textmining
Rhodococcus rhodochrous
-
-
-
Rhodococcus rhodochrous N75
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rhodococcus rhodochrous

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dimethylcatechol + O2 21% of activity with catechol Rhodococcus rhodochrous 3,4-dimethyl-cis,cis-muconate
-
 ?
3,4-dimethylcatechol + O2 21% of activity with catechol Rhodococcus rhodochrous N75 3,4-dimethyl-cis,cis-muconate
-
 ?
3,5-dimethylcatechol + O2 36% of activity with catechol Rhodococcus rhodochrous 3,5-dimethyl-cis,cis-muconate
-
 ?
3,5-dimethylcatechol + O2 36% of activity with catechol Rhodococcus rhodochrous N75 3,5-dimethyl-cis,cis-muconate
-
 ?
3-methylcatechol + O2 64% of activity with catechol Rhodococcus rhodochrous 2-methyl-cis,cis-muconate
-
 ?
3-methylcatechol + O2 64% of activity with catechol Rhodococcus rhodochrous N75 2-methyl-cis,cis-muconate
-
 ?
4-chlorocatechol + O2
-
 Rhodococcus rhodochrous 4-chloro-cis,cis-muconate
-
 ?
4-ethylcatechol + O2 42% of activity with catechol Rhodococcus rhodochrous 4-ethyl-cis-cis-muconate
-
 ?
4-fluorocatechol + O2 65% of activity with catechol Rhodococcus rhodochrous 4-fluoro-cis,cis-muconate
-
 ?
4-iso-propylcatechol + O2 23% of activity with catechol Rhodococcus rhodochrous 4-isopropyl-cis,cis-muconate
-
 ?
4-methylcatechol + O2 76% of activity with catechol Rhodococcus rhodochrous 3-methyl-cis,cis-muconate
-
 ?
4-methylcatechol + O2 76% of activity with catechol Rhodococcus rhodochrous N75 3-methyl-cis,cis-muconate
-
 ?
4-n-propylcatechol + O2 17% of activity with catechol Rhodococcus rhodochrous 4-n-propyl-cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus rhodochrous cis,cis-muconate
-
 ?
catechol + O2
-
 Rhodococcus rhodochrous N75 cis,cis-muconate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 35000, SDS-PAGE Rhodococcus rhodochrous

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 half-life of the enzyme from benzoate-grown cells is 27 min, half-life of the enzyme from p-toluate-grown cells is 14 min Rhodococcus rhodochrous

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 50 mM Bis-Tris-propane buffer and 50 mM Tris-HCl buffer Rhodococcus rhodochrous

pH Range

pH Minimum pH Maximum Comment Organism
7 9 high activity Rhodococcus rhodochrous