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Literature summary for 1.13.11.1 extracted from
- Purification, biochemical properties and substrate specificity of a catechol 1,2-dioxygenase from a phenol degrading Acinetobacter radioresistens (1997), FEBS Lett., 416, 61-64.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0032 | - |
catechol | - |
Acinetobacter radioresistens |  |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Iron | 0.96 mol per mol of protein | Acinetobacter radioresistens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 78000 | 79000 | PAGE, gel filtration | Acinetobacter radioresistens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acinetobacter radioresistens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Acinetobacter radioresistens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 24.5 | - |
- |
Acinetobacter radioresistens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, 100% activity after 1 month | Acinetobacter radioresistens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-2-methylhexa-2,4-dienedioate | - |
? | |
| 4-methylcatechol + O2 | - |
Acinetobacter radioresistens | (2Z,4Z)-3-methylhexa-2,4-dienedioate | - |
? | |
| catechol + O2 | - |
Acinetobacter radioresistens | cis,cis-muconate | - |
? | |
| additional information | both intradiol and extradiol cleavage of 3-methylcatechol | Acinetobacter radioresistens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 38700-39000, at low ionic strength, upon increasing the ionic strength the enzyme monomerizes, SDS-PAGE | Acinetobacter radioresistens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 47 | - |
Acinetobacter radioresistens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 15.8 | - |
catechol | - |
Acinetobacter radioresistens | |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8.5 | the activity rapidly decreases outside this range | Acinetobacter radioresistens |
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