Literature summary for 1.12.99.6 extracted from

Abou Hamdan, A.; Dementin, S.; Liebgott, P.P.; Gutierrez-Sanz, O.; Richaud, P.; De Lacey, A.L.; Rousset, M.; Bertrand, P.; Cournac, L.; Leger, C.
Understanding and tuning the catalytic bias of hydrogenase (2012), J. Am. Chem. Soc., 134, 8368-8371.

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Protein Variants

Protein Variants	Comment	Organism
V74C	moderate increase in the Michaelis constant for H2. The mutant has the same oxidation activity as the wild-type whereas its maximal H2 production rate varies by 2 orders of magnitude	Solidesulfovibrio fructosivorans
V74I	moderate increase in the Michaelis constant for H2	Solidesulfovibrio fructosivorans
V74M	moderate increase in the Michaelis constant for H2, The mutant has the same oxidation activity as the wild-type whereas its maximal H2 production rate varies by 2 orders of magnitude. The ratio of maximal rates for oxidation over production ranges from 2.5 for the wild-type to 200 for the V74M mutant	Solidesulfovibrio fructosivorans

Organism

Organism	UniProt	Comment	Textmining
Solidesulfovibrio fructosivorans	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + methyl viologen	-	Solidesulfovibrio fructosivorans	H+ + reduced methyl viologen	-	?
additional information	the catalytic bias, i.e. the ratio of maximal rates in the two directions is not mainly determined by redox properties of the active site, but rather by steps which occur on sites of the proteins that are remote from the active site	Solidesulfovibrio fructosivorans	?	-	?

Synonyms

Synonyms	Comment	Organism
NiFe hydrogenase	-	Solidesulfovibrio fructosivorans

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Release 2023.1 (January 2023)