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Literature summary for 1.12.99.6 extracted from
- Purification and characterization of membrane-associated hydrogenase from the deep-sea epsilonproteobacterium Hydrogenimonas thermophila (2010), Biosci. Biotechnol. Biochem., 74, 1624-1630.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Hydrogenimonas thermophila | 16020 | - |
| periplasm | localized in both, the periplasmic space and membrane | Hydrogenimonas thermophila | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
SDS-PAGE | Hydrogenimonas thermophila |
| 86300 | - |
gel filtration | Hydrogenimonas thermophila |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenimonas thermophila | - |
- |
- |
| Hydrogenimonas thermophila EPI-55-1%T | - |
- |
- |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| the purified enzyme retains 37% of its initial activity after 72 h of air exposure. After 96 h of air exposure, no activity of the purified enzyme is detected | Hydrogenimonas thermophila |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography, Biogel hydroxyapatite column chromatography, and Superdex 200 gel filtration | Hydrogenimonas thermophila |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 2 | - |
crude extract, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
| 30 | - |
after 152fold purification, at 55°C, pH 7.0, using dichloroindophenol as electron acceptor | Hydrogenimonas thermophila |
| 55 | - |
after 152fold purification, at 55°C, pH 7.0, using methyl viologen as electron acceptor | Hydrogenimonas thermophila |
| 344 | - |
after 152fold purification, at 55°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
| 484 | - |
after 152fold purification, at 80°C, pH 7.0, using methylene blue as electron acceptor | Hydrogenimonas thermophila |
| 1078 | - |
after 152fold purification, at 55°C, pH 7.0, using phenazine methosulfate as electron acceptor | Hydrogenimonas thermophila |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + methyl viologen | - |
Hydrogenimonas thermophila | H+ + reduced methyl viologen | - |
? |  |
| H2 + methyl viologen | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methyl viologen | - |
? | |
| H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila | H+ + reduced dichloroindophenol | - |
? | |
| H2 + oxidized dichloroindophenol | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced dichloroindophenol | - |
? | |
| H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila | H+ + reduced methylene blue | - |
? | |
| H2 + oxidized methylene blue | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced methylene blue | - |
? | |
| H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila | H+ + reduced sodium hydrosulfite | - |
? | |
| H2 + oxidized sodium hydrosulfite | - |
Hydrogenimonas thermophila EPI-55-1%T | H+ + reduced sodium hydrosulfite | - |
? | |
| H2 + phenazine methosulfate | best electron acceptor | Hydrogenimonas thermophila | H+ + reduced phenazine methosulfate | - |
? | |
| additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila | ? | - |
? | |
| additional information | no activity is observed using NAD+, 2,3-dimethoxy methyl-1,4-benzoquinone, and potassium ferricyanide as electron acceptors | Hydrogenimonas thermophila EPI-55-1%T | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| heterodimer | - |
Hydrogenimonas thermophila |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| [NiFe] hydrogenase | - |
Hydrogenimonas thermophila |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
at pH 6.0, using 10 mM methyl viologen or 20 mM sodium hydrosulfite as electron acceptors | Hydrogenimonas thermophila |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 90 | at 90°C the enzyme retains 83% of maximum activity, at lower temperatures hydrogenase activity remains the same as at 10°C (13% of maximum activity), similar activity is observed between 40 and 60°C (about 70% of maximum activity) | Hydrogenimonas thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
50 mM Tris-HCl | Hydrogenimonas thermophila |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10.5 | at pH 9.5 activity decreases to 28% of activity at pH 9.0, hydrogenase activity decreases completely at pH 10.5, while at pH 6.0 45% of activity is retained | Hydrogenimonas thermophila |
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Release 2023.1 (January 2023)
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