Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | 70% of total activity | Alteromonas macleodii | 5737 | - |
membrane | losely membrane-bound, 30% of total activity | Alteromonas macleodii | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | essential element for the assembly and maturation, the addition of Fe-EDTA (0.05 mM) does not affect the level of hydrogenase activity | Alteromonas macleodii | |
additional information | no increase in the hydrogenase activity is observed in response to ferric sulfate, ferric citrate, ferric ammonium citrate, and ferric nitrate | Alteromonas macleodii | |
Ni2+ | essential element for the assembly and maturation, addition of 0.1 mM Ni2+ to the growth medium significantly enhances the hydrogenase activity. Nickel-treatment affects the level of the protein, but not the mRNA | Alteromonas macleodii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alteromonas macleodii | - |
- |
- |
Oxidation Stability | Organism |
---|---|
after exposure to air for 45 days at 4°C, the enzyme retains nearly 100% of its activity when assayed under anaerobic conditions. In 1% O2, 20.4% of its hydrogen-deuterium exchange activity is retained. The accumulation of active HynSL enzyme is enhanced in the presence of molecular oxygen. The activity drops to 5.2% when the reaction is exposed to 3% O2 and no activity is detected in 5% O2 | Alteromonas macleodii |
Purification (Comment) | Organism |
---|---|
DEAE 52 cellulose column chromatography | Alteromonas macleodii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + benzyl viologen | - |
Alteromonas macleodii | H+ + reduced benzyl viologen | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HynSL | - |
Alteromonas macleodii |
[NiFe]-hydrogenase | - |
Alteromonas macleodii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | 80 | the partially purified enzyme retains 94% and 84% of its activity after exposure to 70°C and 80°C for 2 h, respectively | Alteromonas macleodii |