Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.99.6 extracted from

  • Vargas, W.A.; Weyman, P.D.; Tong, Y.; Smith, H.O.; Xu, Q.
    A [NiFe]-hydrogenase from Alteromonas macleodii with unusual stability in the presence of oxygen and high temperature (2011), Appl. Environ. Microbiol., 77, 1990-1998.
    View publication on PubMedView publication on EuropePMC

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm 70% of total activity Alteromonas macleodii 5737
-
membrane losely membrane-bound, 30% of total activity Alteromonas macleodii 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ essential element for the assembly and maturation, the addition of Fe-EDTA (0.05 mM) does not affect the level of hydrogenase activity Alteromonas macleodii
additional information no increase in the hydrogenase activity is observed in response to ferric sulfate, ferric citrate, ferric ammonium citrate, and ferric nitrate Alteromonas macleodii
Ni2+ essential element for the assembly and maturation, addition of 0.1 mM Ni2+ to the growth medium significantly enhances the hydrogenase activity. Nickel-treatment affects the level of the protein, but not the mRNA Alteromonas macleodii

Organism

Organism UniProt Comment Textmining
Alteromonas macleodii
-
-
-

Oxidation Stability

Oxidation Stability Organism
after exposure to air for 45 days at 4°C, the enzyme retains nearly 100% of its activity when assayed under anaerobic conditions. In 1% O2, 20.4% of its hydrogen-deuterium exchange activity is retained. The accumulation of active HynSL enzyme is enhanced in the presence of molecular oxygen. The activity drops to 5.2% when the reaction is exposed to 3% O2 and no activity is detected in 5% O2 Alteromonas macleodii

Purification (Commentary)

Purification (Comment) Organism
DEAE 52 cellulose column chromatography Alteromonas macleodii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + benzyl viologen
-
Alteromonas macleodii H+ + reduced benzyl viologen
-
?

Synonyms

Synonyms Comment Organism
HynSL
-
Alteromonas macleodii
[NiFe]-hydrogenase
-
Alteromonas macleodii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70 80 the partially purified enzyme retains 94% and 84% of its activity after exposure to 70°C and 80°C for 2 h, respectively Alteromonas macleodii