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Literature summary for 1.12.99.6 extracted from

  • Pilet, E.; Nicolet, Y.; Mathevon, C.; Douki, T.; Fontecilla-Camps, J.C.; Fontecave, M.
    The role of the maturase HydG in [FeFe]-hydrogenase active site synthesis and assembly (2009), FEBS Lett., 583, 506-511.
    View publication on PubMed
Search for more literature for 1.12.99.6

Activating Compound

Activating Compound Comment Organism Structure
HydE can coordinate variable amounts of Fe and sulfide Thermotoga maritima
HydE is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
HydEF maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase Chlamydomonas reinhardtii
HydF
-
 Thermotoga maritima
HydF is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
HydG is required to synthesize and assemble the active site utilizing Escherichia coli metabolites Clostridium acetobutylicum
HydG maturase, role in biosynthesis of the active site Desulfovibrio vulgaris
HydG maturase, role in biosynthesis of the active site. HydEF and hydG, coexpressed with the structural genes hydA1 and hydA2 in Escherichia coli lead to active [FeFe]-hydrogenase Chlamydomonas reinhardtii

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Desulfovibrio vulgaris
hydA1 and hydA2 expressed in Escherichia coli Chlamydomonas reinhardtii

Metals/Ions

Metals/Ions Comment Organism Structure
Fe lacks the ferredoxin-like clusters, but contains the H-cluster [Fe4S4] component Chlamydomonas reinhardtii
Fe two ferredoxin-like [Fe4S4] clusters Desulfovibrio vulgaris
Fe [FeS] cluster Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
-
-
Clostridium acetobutylicum
-
-
-
Desulfovibrio vulgaris
-
-
-
Thermotoga maritima
-
-
-

Synonyms

Synonyms Comment Organism
HydA1
-
 Chlamydomonas reinhardtii
HydA2
-
 Chlamydomonas reinhardtii
[FeFe]-hydrogenase
-
 Chlamydomonas reinhardtii
[FeFe]-hydrogenase
-
 Clostridium acetobutylicum
[FeFe]-hydrogenase
-
 Desulfovibrio vulgaris
[FeFe]-hydrogenase
-
 Thermotoga maritima

General Information

General Information Comment Organism
malfunction heterologous expression of the two structural genes of [FeFe]-hydrogenase in Escherichia coli yields an inactive enzyme, only the two ferredoxin-like [Fe4S4] clusters are unambiguously assembled Desulfovibrio vulgaris