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Literature summary for 1.12.99.6 extracted from

  • Bhosale, S.H.; Pant, A.; Khan, M.I.
    Purification and characterization of putative alkaline [Ni-Fe] hydrogenase from unicellular marine green alga, Tetraselmis kochinensis NCIM 1605 (2007), Microbiol. Res., 164, 131-137.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
1-ethyl-3-(3-dimethylaminopropyl) carbodiimide 8 residues modified by 0.5 mM in the presence of 30 mM nitrotyrosine ethylester Tetraselmis sp. KSN-2002
5-5'dithiobis-(2-nitrobenzoate) chemical modification of cysteine residues, 8 residues modified by 0.5 mM. 16 residues modified in the presence of 2% sodium dodecyl sulfate. 20 residues modified by 1 mM and in the presence of urea, EDTA, NaBH4 Tetraselmis sp. KSN-2002

Metals/Ions

Metals/Ions Comment Organism Structure
Fe has Fe-S clusters, contains 10 g atoms of Fe per mol of protein Tetraselmis sp. KSN-2002
Ni contains 1 g of atom of Ni per mol of protein Tetraselmis sp. KSN-2002

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
19000
-
1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002
70000
-
1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002

Organism

Organism UniProt Comment Textmining
Tetraselmis sp. KSN-2002
-
NCIM 1605
-
Tetraselmis sp. KSN-2002 NCIM 1605
-
NCIM 1605
-

Purification (Commentary)

Purification (Comment) Organism
467fold with a yield of 0.13%, to homogeneity, under anaerobic and reducing conditions using sodium dithionite as reducing agent, by ultrafiltration Tetraselmis sp. KSN-2002

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.107
-
crude extract Tetraselmis sp. KSN-2002
50
-
467fold purified enzyme Tetraselmis sp. KSN-2002

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + oxidized methyl viologen
-
Tetraselmis sp. KSN-2002 H+ + reduced methyl viologen
-
?
H2 + oxidized methyl viologen
-
Tetraselmis sp. KSN-2002 NCIM 1605 H+ + reduced methyl viologen
-
?

Subunits

Subunits Comment Organism
dimer 1 * 70000 + 1 * 19000, calculated Tetraselmis sp. KSN-2002

Synonyms

Synonyms Comment Organism
[Ni-Fe] hydrogenase
-
Tetraselmis sp. KSN-2002

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
Tetraselmis sp. KSN-2002

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
Tetraselmis sp. KSN-2002

pI Value

Organism Comment pI Value Maximum pI Value
Tetraselmis sp. KSN-2002 isoelectric focusing
-
9.6