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Literature summary for 1.12.99.6 extracted from

  • Vogt, S.; Lyon, E.J.; Shima, S.; Thauer, R.K.
    The exchange activities of [Fe] hydrogenase (iron-sulfur-cluster-free hydrogenase) from methanogenic archaea in comparison with the exchange activities of [FeFe] and [NiFe] hydrogenases (2008), J. Biol. Inorg. Chem., 13, 97-106.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Methanocaldococcus jannaschii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
methylenetetrahydromethanopterin at pH 6.0 and 25°C Methanocaldococcus jannaschii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methanocaldococcus jannaschii 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron the enzyme harbors an iron-containing cofactor, in which a lowspin iron is complexed by a pyridone, two CO and a cysteine sulfur, [Fe] hydrogenase apoenzyme is converted completely into [Fe] hydrogenase holoenzyme by mixing the apoenzyme with a 3fold excess of the iron-containing [Fe] hydrogenase cofactor Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
2 * 38000, SDS-PAGE Methanocaldococcus jannaschii
76000
-
SDS-PAGE Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
ammonium sulfate precipitation and phenyl-Sepharose column chromatography Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
at pH 6.0 and 25°C Methanocaldococcus jannaschii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + methenyltetrahydromethanopterin the presence of methenyltetrahydromethanopterin is essentially required for H2 activation by [Fe] hydrogenase Methanocaldococcus jannaschii H+ + methylenetetrahydromethanopterin
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 38000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

Synonyms Comment Organism
Hmd
-
Methanocaldococcus jannaschii
hydrogen-forming methylenetetrahydromethanopterin dehydrogenase
-
Methanocaldococcus jannaschii
iron-sulfur-cluster-free hydrogenase
-
Methanocaldococcus jannaschii
[Fe] hydrogenase
-
Methanocaldococcus jannaschii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
65
-
the enzyme is rapidly inactivated at 65°C Methanocaldococcus jannaschii