Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Methanocaldococcus jannaschii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
methylenetetrahydromethanopterin | at pH 6.0 and 25°C | Methanocaldococcus jannaschii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Methanocaldococcus jannaschii | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | the enzyme harbors an iron-containing cofactor, in which a lowspin iron is complexed by a pyridone, two CO and a cysteine sulfur, [Fe] hydrogenase apoenzyme is converted completely into [Fe] hydrogenase holoenzyme by mixing the apoenzyme with a 3fold excess of the iron-containing [Fe] hydrogenase cofactor | Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Methanocaldococcus jannaschii |
76000 | - |
SDS-PAGE | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation and phenyl-Sepharose column chromatography | Methanocaldococcus jannaschii |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
150 | - |
at pH 6.0 and 25°C | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + methenyltetrahydromethanopterin | the presence of methenyltetrahydromethanopterin is essentially required for H2 activation by [Fe] hydrogenase | Methanocaldococcus jannaschii | H+ + methylenetetrahydromethanopterin | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 38000, SDS-PAGE | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
Hmd | - |
Methanocaldococcus jannaschii |
hydrogen-forming methylenetetrahydromethanopterin dehydrogenase | - |
Methanocaldococcus jannaschii |
iron-sulfur-cluster-free hydrogenase | - |
Methanocaldococcus jannaschii |
[Fe] hydrogenase | - |
Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the enzyme is rapidly inactivated at 65°C | Methanocaldococcus jannaschii |