Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cd2+ | reversible inhibition | Lamprobacter modestohalophilus | |
CO | noncompetitive vs. oxidized methyl viologen, competitive vs. H2 | Lamprobacter modestohalophilus | |
Mg2+ | reversible inhibition | Lamprobacter modestohalophilus | |
Ni2+ | competitive vs. Oxidized methyl viologen | Lamprobacter modestohalophilus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Lamprobacter modestohalophilus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
64000 | - |
1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lamprobacter modestohalophilus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Lamprobacter modestohalophilus |
Storage Stability | Organism |
---|---|
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 50 d, approx. 80% loss of activity | Lamprobacter modestohalophilus |
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 50 d, approx. 60% loss of activity | Lamprobacter modestohalophilus |
20°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 50 d, approx. 70% loss of activity | Lamprobacter modestohalophilus |
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under air, 60 d, approx. 40% loss of activity | Lamprobacter modestohalophilus |
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under argon, 60 d, approx. 30% loss of activity | Lamprobacter modestohalophilus |
4°C, 50 mM Tris-HCl, pH 7.0, 0.02% NaN3, storage under H2, 60 d, approx. 50% loss of activity | Lamprobacter modestohalophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + oxidized benzyl viologen | - |
Lamprobacter modestohalophilus | reduced benzyl viologen + H+ | - |
r | |
H2 + oxidized methyl viologen | - |
Lamprobacter modestohalophilus | reduced methyl viologen + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 1 * 64000 + 1 * 30000, SDS-PAGE | Lamprobacter modestohalophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
- |
Lamprobacter modestohalophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 95 | almost no activity at 20°C, approx. 20% of maximal activity at 95°C | Lamprobacter modestohalophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
no loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
85 | - |
approx. 50% loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
100 | - |
almost complete loss of activity after 1.5 min at pH 7.0 | Lamprobacter modestohalophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
H2 evolution | Lamprobacter modestohalophilus |
8.5 | 9.5 | H2 oxidation | Lamprobacter modestohalophilus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.007 | - |
CO | 30°C, pH 7.0 | Lamprobacter modestohalophilus | |
0.16 | - |
Ni2+ | 30°C, pH 9.0 | Lamprobacter modestohalophilus | |
1.35 | - |
Ni2+ | 30°C, pH 7.0 | Lamprobacter modestohalophilus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lamprobacter modestohalophilus | isoelectric focusing | - |
4.5 |