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Literature summary for 1.12.99.6 extracted from
- Molecular cloning, characterization, and overexpression of a novel [Fe]-hydrogenase isolated from a high rate of hydrogen producing Enterobacter cloacae IIT-BT 08 (2004), Biochem. Biophys. Res. Commun., 324, 679-685.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli as GST-tagged enzyme | Enterobacter cloacae |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | Fe-hydrogenase | Enterobacter cloacae |  |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16000 | - |
x * 16000, deduced from nucleotide sequence | Enterobacter cloacae |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Enterobacter cloacae | - |
IIT-BT 08 | - |
| Enterobacter cloacae IIT-BT 08 | - |
IIT-BT 08 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme | Enterobacter cloacae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + oxidized methyl viologen | - |
Enterobacter cloacae | reduced methyl viologen + H+ | - |
r | |
| H2 + oxidized methyl viologen | - |
Enterobacter cloacae IIT-BT 08 | reduced methyl viologen + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16000, deduced from nucleotide sequence | Enterobacter cloacae |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| hydA | - |
Enterobacter cloacae |
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