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Literature summary for 1.12.99.6 extracted from
- Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction (2000), J. Bacteriol., 182, 1864-1871.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | Km for various electron donors | Pyrococcus furiosus | |
| 0.128 | - |
NAD+ | - |
Pyrococcus furiosus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe | 21 atoms per mol enzyme, 5 4Fe-4S cluster and 1 2Fe-2S cluster | Pyrococcus furiosus | |
| Ni | 0.9 atoms per mol enzyme | Pyrococcus furiosus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 24000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 30000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 39000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 52000 | - |
alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
| 320000 | - |
gel filtration | Pyrococcus furiosus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus furiosus | - |
strain DSM 3638 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Pyrococcus furiosus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
specific activities with various electron donors | Pyrococcus furiosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | benzyl viologen, methyl viologen, NADPH and NADH can serve as electron donors | Pyrococcus furiosus | H+ + reduced acceptor | - |
? | |
| H2 + NAD+ | - |
Pyrococcus furiosus | NADH + H+ | - |
r | |
| H2 + S | - |
Pyrococcus furiosus | H2S | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | alpha,beta,gamma,delta, 1 * 52000 + 1 * 39000 + 1 * 30000 + 1 * 24000, SDS-PAGE | Pyrococcus furiosus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | turnover number for various electron donors | Pyrococcus furiosus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | 0.83 mol FAD per enzyme | Pyrococcus furiosus | |
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Release 2023.1 (January 2023)
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