Any feedback?
Literature summary for 1.12.99.6 extracted from
- Characterization and thermostability of a membrane-bound hydrogenase from a thermophilic hydrogen oxidizing bacterium, Bacillus schlegelii (1993), Biosci. Biotechnol. Biochem., 57, 1177-1179.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | complete inhibition at 1 mM | Hydrogenibacillus schlegelii |  |
| Hg2+ | complete inhibition at 1 mM | Hydrogenibacillus schlegelii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ni | NiFe-hydrogenase | Hydrogenibacillus schlegelii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
| 66000 | - |
1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
| 100000 | - |
gel filtration | Hydrogenibacillus schlegelii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Hydrogenibacillus schlegelii | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Hydrogenibacillus schlegelii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.4 | 0.7 | H2 evolution | Hydrogenibacillus schlegelii |
| 84 | - |
H2 oxidation | Hydrogenibacillus schlegelii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| H2 + acceptor | methylene blue acts as electron acceptor | Hydrogenibacillus schlegelii | H+ + reduced acceptor | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 1 * 66000 + 1 * 28000, SDS-PAGE | Hydrogenibacillus schlegelii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Hydrogenibacillus schlegelii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
90% activity after 12 h | Hydrogenibacillus schlegelii |
| 70 | - |
50% activity after 4 h | Hydrogenibacillus schlegelii |
| 80 | - |
50% activity after 2.5 h | Hydrogenibacillus schlegelii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
