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Literature summary for 1.12.98.1 extracted from

  • Kulkarni, G.; Kridelbaugh, D.; Guss, A.; Metcalf, W.
    Hydrogen is a preferred intermediate in the energy-conserving electron transport chain of Methanosarcina barkeri (2009), Proc. Natl. Acad. Sci. USA, 106, 15915-15920.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methanosarcina barkeri

Protein Variants

Protein Variants Comment Organism
additional information Methanosarcina barkeri Delta fpo mutants: strain WWM85 Delta hpt::PmcrB-Phi C31int-attP, strain WWM86 Delta hpt::PmcrB-Phi C31int-attB, strain WWM71 Delta hpt::PmcrB-Phi C31int-attB, Delta fpoA-O Deletion of fpo by markerless exchange with pDK4 in WWM86, strain WWM123 Delta hpt::PmcrB-Phi C31int-attP, Delta fpoF Deletion of fpoF by markerless exchange with pDK13 in WWM85, strain WWM116 Delta hpt::PmcrB-Phi C31int-attP, Delta freAEGB Deletion of fre by markerless exchange with pGK6 in WWM85, strain WWM122 Delta hpt::PmcrB-Phi C31int-attB, Delta frhADGB::pac-hpt Deletion of frh with ApaI/NotI-digested pAMG81 in WWM86, strain WWM108 Delta hpt::PmcrB-Phi C31int-attB, Delta fpoA-O, Delta frhADGB::pac-hpt Deletion of frh with ApaI/NotI-digested pAMG81 in WWM71, strain WWM145 Delta hpt::PmcrB-Delta C31int-attP, Delta fpoF, Delta frhADGB::pac-hpt Deletion of frh with ApaI/NotI-digested pAMG81 in WWM123 Methanosarcina barkeri

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Methanosarcina barkeri 5737
-

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri
-
-
-

Synonyms

Synonyms Comment Organism
F420-reducing hydrogenase
-
Methanosarcina barkeri

General Information

General Information Comment Organism
malfunction loss of F420H2 dehydrogenase, and therefore of the 420H2:heterodisulfide oxidoreductase system, does not measurably affect methanogenesis or growth in Methanosarcina barkeri Methanosarcina barkeri
metabolism the preferred electron transport chain involves production of hydrogen gas in the cytoplasm, which then diffuses out of the cell, where it is reoxidized with transfer of electrons into the energy-conserving electron transport chain. This hydrogen-cycling metabolism leads directly to production of a proton motive force that can be used by the cell for ATP synthesis Methanosarcina barkeri