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Literature summary for 1.12.98.1 extracted from

  • Baron, S.F.; Ferry, J.G.
    Purification and properties of the membrane-associated coenzyme F420-reducing hydrogenase from Methanobacterium formicicum (1989), J. Bacteriol., 171, 3846-3853.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.98.1

Activating Compound

Activating Compound Comment Organism Structure
FAD reactivation without added FAD restores only methyl viologen-dependent activity, no activity with F420 Methanobacterium formicicum
KCl 0.8 M activate F420-linked activity more than 10fold, slight inhibition of methyl viologen linked activity Methanobacterium formicicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.037
-
 coenzyme F420
-
 Methanobacterium formicicum
0.08
-
 coenzyme F0
-
 Methanobacterium formicicum
0.087
-
 FAD
-
 Methanobacterium formicicum
1.56
-
 methyl viologen
-
 Methanobacterium formicicum

Metals/Ions

Metals/Ions Comment Organism Structure
Iron
-
 Methanobacterium formicicum
Nickel
-
 Methanobacterium formicicum
selenium no selenium detected Methanobacterium formicicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102000
-
 smallest active species, gel filtration Methanobacterium formicicum
109000
-
 gel filtration Methanobacterium formicicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + coenzyme F420 Methanobacterium formicicum enzyme of methanogenesis pathway reduced coenzyme F420
-
 r

Organism

Organism UniProt Comment Textmining
Methanobacterium formicicum
-
-
-

Oxidation Stability

Oxidation Stability Organism
purified enzyme requires reductive reactivation before assay, reactivation possible with H2 or glucose and glucose oxidase Methanobacterium formicicum

Purification (Commentary)

Purification (Comment) Organism
-
 Methanobacterium formicicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
97.6
-
 purified enzyme with F420 Methanobacterium formicicum
98.6
-
 purified enzyme with F0 Methanobacterium formicicum
172
-
 purified enzyme with methyl viologen Methanobacterium formicicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + coenzyme F0
-
 Methanobacterium formicicum reduced coenzyme F0
-
 ?
H2 + coenzyme F420
-
 Methanobacterium formicicum reduced coenzyme F420
-
 r
H2 + coenzyme F420 enzyme of methanogenesis pathway Methanobacterium formicicum reduced coenzyme F420
-
 r
H2 + FAD
-
 Methanobacterium formicicum H+ + FADH2
-
 r
H2 + methyl viologen
-
 Methanobacterium formicicum ?
-
 ?
additional information reduces different flavins Methanobacterium formicicum ?
-
 ?

Subunits

Subunits Comment Organism
multimer alpha,beta,gamma 43600 + 36700 + 28000, SDS-PAGE, exact subunit composition not known, ratio 1:1:1 postulated Methanobacterium formicicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Methanobacterium formicicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7 7.5
-
 Methanobacterium formicicum

pH Range

pH Minimum pH Maximum Comment Organism
5.5 9.5
-
 Methanobacterium formicicum
5.5 8 reverse reaction Methanobacterium formicicum

Cofactor

Cofactor Comment Organism Structure
FAD 1 mol per mol 109000 Da species Methanobacterium formicicum
FAD FAD depleted enzyme reduces methyl viologen but not F420 Methanobacterium formicicum
additional information no activity with FMN after FAD depletion Methanobacterium formicicum