Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.98.1 extracted from

  • Sprott, G.D.; Shaw, K.M.; Beveridge, T.J.
    Properties of the particulate enzyme F420-reducing hydrogenase isolated from Methanospirillum hungatei (1987), Can. J. Microbiol., 33, 896-904.
No PubMed abstract available
Search for more literature for 1.12.98.1

General Stability

General Stability Organism
freezing and thawing changes properties of enzyme, different behavior in anion exchange chromatography Methanospirillum hungatei
unstable in prereduced buffer, dramatic decline of activity after 2 h Methanospirillum hungatei

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activating Methanospirillum hungatei
Iron
-
 Methanothermobacter thermautotrophicus
Iron non-heme iron Methanospirillum hungatei
Mg2+ activating Methanospirillum hungatei
Nickel
-
 Methanothermobacter thermautotrophicus
Nickel
-
 Methanospirillum hungatei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30700
-
 alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei
50700
-
 alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei
720000
-
-
 Methanospirillum hungatei
960000
-
 gel filtration Methanothermobacter thermautotrophicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + coenzyme F420 Methanothermobacter thermautotrophicus enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?
H2 + coenzyme F420 Methanospirillum hungatei enzyme of methanogenesis pathway reduced coenzyme F420
-
 ?

Organism

Organism UniProt Comment Textmining
Methanospirillum hungatei
-
-
-
Methanothermobacter thermautotrophicus
-
 strain delta H
-

Purification (Commentary)

Purification (Comment) Organism
-
 Methanothermobacter thermautotrophicus
-
 Methanospirillum hungatei

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.847
-
 purified enzyme, F420-linked activity Methanospirillum hungatei
11.1
-
 purified enzyme, benzyl viologen-linked activity Methanospirillum hungatei

Storage Stability

Storage Stability Organism
liquid nitrogen, in presence of 50% sucrose and 10% glycerol, stable Methanospirillum hungatei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + benzyl viologen
-
 Methanospirillum hungatei ?
-
 ?
H2 + coenzyme F420
-
 Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
H2 + coenzyme F420
-
 Methanospirillum hungatei reduced coenzyme F420
-
 ?
H2 + coenzyme F420 enzyme of methanogenesis pathway Methanothermobacter thermautotrophicus reduced coenzyme F420
-
 ?
H2 + coenzyme F420 enzyme of methanogenesis pathway Methanospirillum hungatei reduced coenzyme F420
-
 ?

Subunits

Subunits Comment Organism
multimer alpha,beta,gamma 45000 + 31000 + 28000, SDS-PAGE, exact subunit composition not known Methanothermobacter thermautotrophicus
multimer alpha,beta 5 * 50700 + 15 * 30700, SDS-PAGE, electron microscopy Methanospirillum hungatei

Cofactor

Cofactor Comment Organism Structure
additional information no requirement for added FAD Methanospirillum hungatei