Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Chlamydomonas reinhardtii | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Tetradesmus obliquus | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Clostridium pasteurianum | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | [Chlorella] fusca | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Megasphaera elsdenii | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Desulfovibrio desulfuricans | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Clostridium acetobutylicum | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Chlamydomonas moewusii | |
Fe2+ | the protein contains iron but no nickel, the active site cofactor is a unique [Fe-S] compound commonly referred to as H-cluster | Chlorococcum submarinum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
14000 | - |
1 * 46100 + 1 * 14000, calculated from amino acid sequence | Desulfovibrio desulfuricans |
44600 | - |
calculated from amino acid sequence | Tetradesmus obliquus |
45100 | - |
calculated from amino acid sequence | [Chlorella] fusca |
45300 | - |
calculated from amino acid sequence | Chlorococcum submarinum |
45400 | - |
calculated from amino acid sequence | Chlamydomonas moewusii |
46100 | - |
1 * 46100 + 1 * 14000, calculated from amino acid sequence | Desulfovibrio desulfuricans |
47300 | - |
isozyme HydA2, calculated from amino acid sequence | Chlamydomonas reinhardtii |
47500 | - |
isozyme HydA1, calculated from amino acid sequence | Chlamydomonas reinhardtii |
48000 | - |
isozyme HydA1, SDS-PAGE | Chlamydomonas reinhardtii |
53600 | - |
calculated from amino acid sequence | Megasphaera elsdenii |
63800 | - |
calculated from amino acid sequence | Clostridium pasteurianum |
64300 | - |
calculated from amino acid sequence | Clostridium acetobutylicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + reduced ferredoxin | Chlamydomonas reinhardtii | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Tetradesmus obliquus | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Clostridium pasteurianum | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | [Chlorella] fusca | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Megasphaera elsdenii | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Desulfovibrio desulfuricans | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Clostridium acetobutylicum | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Chlamydomonas moewusii | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | Chlorococcum submarinum | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | H2 + oxidized ferredoxin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlamydomonas moewusii | - |
- |
- |
Chlamydomonas reinhardtii | - |
- |
- |
Chlorococcum submarinum | - |
- |
- |
Clostridium acetobutylicum | - |
- |
- |
Clostridium pasteurianum | - |
- |
- |
Desulfovibrio desulfuricans | - |
- |
- |
Megasphaera elsdenii | - |
- |
- |
Tetradesmus obliquus | - |
- |
- |
[Chlorella] fusca | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity column chromatography with immobilised ferredoxin | Chlamydomonas reinhardtii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Chlamydomonas reinhardtii | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Tetradesmus obliquus | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Clostridium pasteurianum | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | [Chlorella] fusca | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Megasphaera elsdenii | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Desulfovibrio desulfuricans | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Clostridium acetobutylicum | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Chlamydomonas moewusii | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced ferredoxin | hydrogen production is light-dependent, since the [FeFe] hydrogenase is coupled to the photosynthetic electron transport chain via ferredoxin | Chlorococcum submarinum | H2 + oxidized ferredoxin | - |
? | |
H+ + reduced methyl viologen | - |
Chlamydomonas reinhardtii | H2 + oxidized methyl viologen | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterodimer | 1 * 46100 + 1 * 14000, calculated from amino acid sequence | Desulfovibrio desulfuricans |
Synonyms | Comment | Organism |
---|---|---|
CpI | - |
Clostridium pasteurianum |
DDH | - |
Desulfovibrio desulfuricans |
hydA | - |
Tetradesmus obliquus |
hydA | - |
[Chlorella] fusca |
hydA | - |
Megasphaera elsdenii |
hydA | - |
Clostridium acetobutylicum |
hydA | - |
Chlorococcum submarinum |
HydA1 | - |
Chlamydomonas moewusii |
HydA1 | isozyme | Chlamydomonas reinhardtii |
HydA2 | isozyme | Chlamydomonas reinhardtii |
hydrogenase of the Fe-only type | - |
Chlamydomonas reinhardtii |
[FeFe] hydrogenase | - |
Chlamydomonas reinhardtii |
[FeFe] hydrogenase | - |
Tetradesmus obliquus |
[FeFe] hydrogenase | - |
Clostridium pasteurianum |
[FeFe] hydrogenase | - |
[Chlorella] fusca |
[FeFe] hydrogenase | - |
Megasphaera elsdenii |
[FeFe] hydrogenase | - |
Desulfovibrio desulfuricans |
[FeFe] hydrogenase | - |
Clostridium acetobutylicum |
[FeFe] hydrogenase | - |
Chlamydomonas moewusii |
[FeFe] hydrogenase | - |
Chlorococcum submarinum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
Ferredoxin | - |
Chlamydomonas reinhardtii | |
Ferredoxin | - |
Tetradesmus obliquus | |
Ferredoxin | - |
Clostridium pasteurianum | |
Ferredoxin | - |
[Chlorella] fusca | |
Ferredoxin | - |
Megasphaera elsdenii | |
Ferredoxin | - |
Desulfovibrio desulfuricans | |
Ferredoxin | - |
Clostridium acetobutylicum | |
Ferredoxin | - |
Chlamydomonas moewusii | |
Ferredoxin | - |
Chlorococcum submarinum |
Organism | Comment | Expression |
---|---|---|
Chlamydomonas reinhardtii | activation and de novo synthesis of the protein is inhibited by cycloheximide but not chloramphenicol | up |