Literature summary for 1.12.7.2 extracted from

Long, H.; King, P.W.; Ghirardi, M.L.; Kim, K.
Hydrogenase/ferredoxin charge-transfer complexes: effect of hydrogenase mutations on the complex association (2009), J. Phys. Chem. A, 113, 4060-4067.

Search for more literature for 1.12.7.2

Protein Variants

Protein Variants	Comment	Organism
D102K	leads to lower binding free energies and higher association rate with [2Fe2S]-ferredoxin FDX1 and is thus a promising target for improving hydrogen production rates in engineered organisms	Chlamydomonas reinhardtii
D102K/E221K	double mutant enhances association rate constant	Chlamydomonas reinhardtii
D102K/T99K	double mutant enhances association rate constant	Chlamydomonas reinhardtii
E221K	higher association rate with [2Fe2S]-ferredoxin FDX1	Chlamydomonas reinhardtii
M214K	least enhancement of association rate with [2Fe2S]-ferredoxin FDX1, destabilizes binding complexes	Chlamydomonas reinhardtii
T99K	leads to lower binding free energies and higher association rate with [2Fe2S]-ferredoxin FDX1 and is thus a promising target for improving hydrogen production rates in engineered organisms	Chlamydomonas reinhardtii

Organism

Organism	UniProt	Comment	Textmining
Chlamydomonas reinhardtii	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
H2 + oxidized ferredoxin	-	Chlamydomonas reinhardtii	reduced ferredoxin + 2 H+	-	?

Synonyms

Synonyms	Comment	Organism
HydA2	-	Chlamydomonas reinhardtii
[FeFe]-hydrogenase	-	Chlamydomonas reinhardtii

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Chlamydomonas reinhardtii

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Release 2023.1 (January 2023)