Inhibitors | Comment | Organism | Structure |
---|---|---|---|
O2 | 50% loss of H2 evolution activity after 3 h | Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Pyrococcus furiosus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | 4 Fe-atoms per mol hydrogenase | Pyrococcus furiosus | |
Ni | 1 Ni atom per mol, redox active Ni-site | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
20000 | - |
alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
40000 | - |
alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Oxidation Stability | Organism |
---|---|
rapid inactivation under aerobic conditions | Pyrococcus furiosus |
Purification (Comment) | Organism |
---|---|
under anaerobic conditions, solubilized with n-dodecyl-beta-D-maltoside | Pyrococcus furiosus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
hydrogen oxidation, benzyl viologen as electron acceptor | Pyrococcus furiosus |
9.4 | - |
hydrogen evolution, reduced methyl viologen as electron donor | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | benzyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | NADP+ as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
additional information | ferredoxin is not an efficient electron carrier for both hydrogenases | Pyrococcus furiosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | alpha, beta, 1 * 40000 + 1 * 20000, SDS-PAGE | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
100 | - |
50% inactivation after 30 min | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Pyrococcus furiosus |