KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.017 | - |
NADP+ | - |
Pyrococcus furiosus | |
0.07 | - |
NADPH | - |
Pyrococcus furiosus | |
0.07 | - |
NADH | - |
Pyrococcus furiosus | |
0.125 | - |
NAD+ | - |
Pyrococcus furiosus | |
0.2 | - |
sulfur | - |
Pyrococcus furiosus | |
1 | - |
methyl viologen | at 120 kPa H2 | Pyrococcus furiosus | |
1.25 | - |
reduced methyl viologen | - |
Pyrococcus furiosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Pyrococcus furiosus | 5737 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe | five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit | Pyrococcus furiosus | |
Fe | 21 atoms per mol heterotetramer | Pyrococcus furiosus | |
Ni | 0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit | Pyrococcus furiosus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
30000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
39000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
52000 | - |
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
320000 | - |
gel filtration | Pyrococcus furiosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
strain DSM 3638 | - |
Pyrococcus furiosus | - |
hydrogenase II | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.02 | - |
hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II | Pyrococcus furiosus |
0.2 | - |
sulfur reduction, hydrogenase II | Pyrococcus furiosus |
0.98 | - |
hydrogen production, sodium dithionite as electron donor, hydrogenase II | Pyrococcus furiosus |
11 | - |
hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II | Pyrococcus furiosus |
40 | - |
hydrogen production, methyl viologen as electron donor, hydrogenase II | Pyrococcus furiosus |
131 | - |
hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II | Pyrococcus furiosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H+ + NADH | - |
Pyrococcus furiosus | H2 + NAD+ | - |
r | |
H+ + reduced methyl viologen | - |
Pyrococcus furiosus | H2 + oxidized methyl viologen | - |
? | |
H2 + electron acceptor | methyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | benzyl viologen as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + electron acceptor | NADP+ as electron acceptor | Pyrococcus furiosus | H+ + reduced electron acceptor | - |
r | |
H2 + NAD+ | - |
Pyrococcus furiosus | H+ + NADH | - |
r | |
additional information | ferredoxin is not an efficient electron carrier for both hydrogenases | Pyrococcus furiosus | ? | - |
? | |
S + NADPH | - |
Pyrococcus furiosus | H2S + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
octamer | alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
90 | - |
above 90°C, hydrogenase II | Pyrococcus furiosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
50% inactivation after 6 h, hydrogenase II | Pyrococcus furiosus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus | |
FAD | hydrogenase II, 0.83 mol per mol heterotetramer | Pyrococcus furiosus | |
NADH | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus | |
NADPH | putative nucleotide-binding site in the gamma subunit | Pyrococcus furiosus |