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Literature summary for 1.12.7.2 extracted from

  • Ma, K.; Weiss, R.; Adams, M.W.W.
    Characterization of hydrogenase II from the hyperthermophilic archaeon Pyrococcus furiosus and assessment of its role in sulfur reduction (2000), J. Bacteriol., 182, 1864-1871.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
NADP+
-
Pyrococcus furiosus
0.07
-
NADPH
-
Pyrococcus furiosus
0.07
-
NADH
-
Pyrococcus furiosus
0.125
-
NAD+
-
Pyrococcus furiosus
0.2
-
sulfur
-
Pyrococcus furiosus
1
-
methyl viologen at 120 kPa H2 Pyrococcus furiosus
1.25
-
reduced methyl viologen
-
Pyrococcus furiosus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Pyrococcus furiosus 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Fe five [4Fe4S] clusters, 2 in the beta subunit and 3 in the delta subunit, additionally one [2Fe2S] cluster in the gamma subunit Pyrococcus furiosus
Fe 21 atoms per mol heterotetramer Pyrococcus furiosus
Ni 0.9 atoms per mol heterotetramer, NiFe-catalytic site in the alpha-subunit Pyrococcus furiosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
24000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
30000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
39000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
52000
-
alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus
320000
-
gel filtration Pyrococcus furiosus

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus
-
strain DSM 3638
-
Pyrococcus furiosus
-
hydrogenase II
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.02
-
hydrogen production, oxidized ferredoxin as electron donor, hydrogenase II Pyrococcus furiosus
0.2
-
sulfur reduction, hydrogenase II Pyrococcus furiosus
0.98
-
hydrogen production, sodium dithionite as electron donor, hydrogenase II Pyrococcus furiosus
11
-
hydrogen oxidation, methyl viologen as electron acceptor, hydrogenase II Pyrococcus furiosus
40
-
hydrogen production, methyl viologen as electron donor, hydrogenase II Pyrococcus furiosus
131
-
hydrogen oxidation, benzyl viologen as electron acceptor, hydrogenase II Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H+ + NADH
-
Pyrococcus furiosus H2 + NAD+
-
r
H+ + reduced methyl viologen
-
Pyrococcus furiosus H2 + oxidized methyl viologen
-
?
H2 + electron acceptor methyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
r
H2 + electron acceptor benzyl viologen as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
r
H2 + electron acceptor NADP+ as electron acceptor Pyrococcus furiosus H+ + reduced electron acceptor
-
r
H2 + NAD+
-
Pyrococcus furiosus H+ + NADH
-
r
additional information ferredoxin is not an efficient electron carrier for both hydrogenases Pyrococcus furiosus ?
-
?
S + NADPH
-
Pyrococcus furiosus H2S + NADP+
-
?

Subunits

Subunits Comment Organism
octamer alpha, beta, gamma, delta, 2 * 52000 + 2 * 39000 + 2 * 30000 + 2 * 24000, SDS-PAGE Pyrococcus furiosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
90
-
above 90°C, hydrogenase II Pyrococcus furiosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
50% inactivation after 6 h, hydrogenase II Pyrococcus furiosus

Cofactor

Cofactor Comment Organism Structure
FAD putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus
FAD hydrogenase II, 0.83 mol per mol heterotetramer Pyrococcus furiosus
NADH putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus
NADPH putative nucleotide-binding site in the gamma subunit Pyrococcus furiosus