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Literature summary for 1.12.2.1 extracted from

  • Rashamuse, K.; Mutambanengwe, C.; Whiteley, C.
    Enzymatic recovery of platinum (IV) from industrial wastewater using a biosulphidogenic hydrogenase (2008), Afr. J. Biotechnol., 7, 1087-1095.
No PubMed abstract available
Search for more literature for 1.12.2.1

Application

Application Comment Organism
industry platinum (IV) recovery from an industrial effluent through a biosulphidogenic sulphate reducing bacteria consortium via a hydrogenase-cytochrome c3 enzyme system that removes electrons from hydrogen to the platinum metal that acts as a final electron acceptor Bacteria

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 1 mM, 97% inhibition Bacteria
Cr6+ 1 mM, complete inhibition Bacteria
Cu2+ 1 mM, 95% inhibition Bacteria
Zn2+ 1 mM, 79% inhibition Bacteria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0052
-
 methyl viologen
-
 Bacteria

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Bacteria 16020
-

Metals/Ions

Metals/Ions Comment Organism Structure
Iron increases activity by 1.8% Bacteria
Nickel increases activity by 1.6% Bacteria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
 x * 58000, SDS-PAGE Bacteria

Organism

Organism UniProt Comment Textmining
Bacteria
-
 sulphate reducing bacteria
-

Purification (Commentary)

Purification (Comment) Organism
by sonication, PEG 20000 concentration, ion exchange chromatography and gel filtration, 9.26fold purified with a yield of 51.8% Bacteria

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3817
-
 crude sonicate Bacteria
35340
-
 9.26fold purified Bacteria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ferricytochrome c3 + 2 H2 48% relative activity compared to activity with oxidized methyl viologen Bacteria ferrocytochrome c3 + 4 H+
-
 ?
H2 + oxidized dichloroindophenol 30% relative activity compared to activity with oxidized methyl viologen Bacteria H+ + ?
-
 ?
H2 + oxidized methyl viologen 100% relative activity Bacteria H+ + reduced methyl viologen
-
 ?
H2 + potassium ferricyanide 34% relative activity compared to activity with oxidized methyl viologen Bacteria H+ + ?
-
 ?
additional information hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3 Bacteria ?
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, SDS-PAGE Bacteria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35 40
-
 Bacteria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
35 40 at optimal temperature range of 35-40°C, a minimal activity loss during the first 10 to 20 min and thereafter, activity steadily decreases as incubation time increases. After 80 min of incubation, the enzyme loses 75-80% of all of its activity Bacteria
55
-
 completely denatured above Bacteria

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 methyl viologen
-
 Bacteria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Bacteria

Cofactor

Cofactor Comment Organism Structure
cytochrome c3 hydrogen-dependent platinum (IV) reducing activity in the presence of hydrogenase and its physiological electron carrier, cytochrome c3. Reduction of platinum by the purified hydrogenase enzyme alone yields about 10% removal after 3 h incubation while after the addition of cytochrome c3 there is a slight increase to 15%. Suppression of enzyme activity due to the extreme acid pH of 0.38 of the effluent Bacteria