Literature summary for 1.12.1.2 extracted from

Lauterbach, L.; Liu, J.; Horch, M.; Hummel, P.; Schwarze, A.; Haumann, M.; Vincent, K.; Lenz, O.; Zebger, I.
The hydrogenase subcomplex of the NAD+-reducing [NiFe] hydrogenase from Ralstonia eutropha - Insights into catalysis and redox interconversions (2011), Eur. J. Inorg. Chem., 2011, 1067-1079.

No PubMed abstract available

Search for more literature for 1.12.1.2

Activating Compound

Activating Compound	Comment	Organism	Structure
FMN	H2 oxidation by subunits HoxHY is enhanced on addition of excess FMN	Cupriavidus necator

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Cupriavidus necator	Cupriavidus necator

Inhibitors

Inhibitors	Comment	Organism	Structure
O2	incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2; incubation of subunits HoxHY with O2 at high potentials causes slow inactivation, but activity is recovered within seconds at potentials below -170 mV at 30°C, even in the presence of 2% O2	Cupriavidus necator

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Iron	the active site iron atom has a standard ligation, i.e., one CO and two cyanide ligands	Cupriavidus necator

Organism

Organism	UniProt	Comment	Textmining
Cupriavidus necator	P22319	subunit delta	-
Cupriavidus necator	P22320	subunit beta	-
Cupriavidus necator DSM 428	P22319	subunit delta	-
Cupriavidus necator DSM 428	P22320	subunit beta	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant protein. The as-isolated module HoxHY is initially catalytically inactive, but after reductive activation at low potentials, exhibits both H2 oxidation and H+ reduction	Cupriavidus necator

Synonyms

Synonyms	Comment	Organism
HoxH	-	Cupriavidus necator
HoxS	-	Cupriavidus necator

Cofactor

Cofactor	Comment	Organism	Structure
4Fe-4S-center	presence of a 4Fe-4S-center in addition to the active site iron	Cupriavidus necator
FMN	cofactor and stabilization of the active site	Cupriavidus necator

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Release 2023.1 (January 2023)