Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.12.1.2 extracted from

  • Rakhely, G.; Kovacs, A.T.; Maroti, G.; Fodor, B.D.; Csanadi, G.; Latinovics, D.; Kovacs, K.L.
    Cyanobacterial-type, heteropentameric, NAD+-reducing NiFe hydrogenase in the purple sulfur photosynthetic bacterium Thiocapsa roseopersicina (2004), Appl. Environ. Microbiol., 70, 722-728.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.12.1.2

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, HoxE is encoded in a cluster of 5 genes encoding the enzyme complex subunits, sigma54-type promotor with probably inducible expression of the operon Thiocapsa roseopersicina

Protein Variants

Protein Variants Comment Organism
additional information in-frame deletion of HoxE, deletion of HoxH Thiocapsa roseopersicina

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Thiocapsa roseopersicina 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
H2 + NAD+ Thiocapsa roseopersicina in vivo H2 evolution under nitrogenase-repressed conditions H+ + NADH
-
 r

Organism

Organism UniProt Comment Textmining
Thiocapsa roseopersicina Q6XQK5 purple sulfur photosynthetic bacterium
-

Purification (Commentary)

Purification (Comment) Organism
partially Thiocapsa roseopersicina

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 determination of H2 production rates in vivo Thiocapsa roseopersicina

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
H2 + NAD+
-
 Thiocapsa roseopersicina H+ + NADH
-
 r
H2 + NAD+ in vivo H2 evolution under nitrogenase-repressed conditions Thiocapsa roseopersicina H+ + NADH
-
 r
H2 + oxidized methyl viologen
-
 Thiocapsa roseopersicina H+ + reduced methyl viologen
-
 r

Subunits

Subunits Comment Organism
More the subunit HoxE, which is essential for the bidirectional hydrogenase activity, is included in a pentameric bidirectional hydrogenase complex HoxEFUYH of the cyanobacterial-type, complex components are HoxE, HoxF, HoxH, HoxU, and HoxY Thiocapsa roseopersicina

Synonyms

Synonyms Comment Organism
Hox
-
 Thiocapsa roseopersicina
NAD+-reducing NiFe hydrogenase
-
 Thiocapsa roseopersicina
NiFe hydrogenase
-
 Thiocapsa roseopersicina

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at, H2 evolution Thiocapsa roseopersicina

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thiocapsa roseopersicina
NADH
-
 Thiocapsa roseopersicina