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Literature summary for 1.12.1.2 extracted from

  • Grzeszik, C.; Ross, K.; Schneider, K.; Reh, M.; Schlegel, H.G.
    Location, catalytic activity, and subunit composition of NAD-reducing hydrogenases of some Alcaligenes strains and Rhodococcus opacus MR22 (1997), Arch. Microbiol., 167, 172-176.
    View publication on PubMed
Search for more literature for 1.12.1.2

Organism

Organism UniProt Comment Textmining
Achromobacter denitrificans
-
-
-
Achromobacter denitrificans 4a-2
-
-
-
Achromobacter ruhlandii
-
-
-
Cupriavidus necator
-
-
-
Cupriavidus necator Cd2/01
-
-
-
Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1
-
-
-
Rhodococcus opacus
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6
-
 strain Cd2/01, electron acceptor NAD+ Cupriavidus necator
7.7
-
 electron acceptor NAD+ Achromobacter denitrificans
34
-
 electron acceptor NAD+ Achromobacter ruhlandii
38
-
 strain H16, electron acceptor NAD+ Cupriavidus necator
72
-
 electron acceptor NAD+ Rhodococcus opacus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 ferricyanide + NAD(P)H
-
 Cupriavidus necator 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Rhodococcus opacus 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Achromobacter denitrificans 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Achromobacter ruhlandii 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Cupriavidus necator Cd2/01 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Achromobacter denitrificans 4a-2 2 ferrocyanide + NAD(P)+ + H+
-
 ?
2 ferricyanide + NAD(P)H
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 2 ferrocyanide + NAD(P)+ + H+
-
 ?
H2 + ferricyanide 1257% activity compared to electron acceptor NAD+ Achromobacter denitrificans H+ + ferrocyanide
-
 ?
H2 + ferricyanide 350% activity compared to electron acceptor NAD+ Rhodococcus opacus H+ + ferrocyanide
-
 ?
H2 + ferricyanide 585% activity compared to electron acceptor NAD+ Achromobacter denitrificans H+ + ferrocyanide
-
 ?
H2 + ferricyanide 708% activity compared to electron acceptor NAD+ Cupriavidus necator H+ + ferrocyanide
-
 ?
H2 + ferricyanide 389% activity compared to electron acceptor NAD+ Achromobacter ruhlandii H+ + ferrocyanide
-
 ?
H2 + ferricyanide 708% activity compared to electron acceptor NAD+ Cupriavidus necator Cd2/01 H+ + ferrocyanide
-
 ?
H2 + ferricyanide 1257% activity compared to electron acceptor NAD+ Achromobacter denitrificans 4a-2 H+ + ferrocyanide
-
 ?
H2 + ferricyanide 708% activity compared to electron acceptor NAD+ Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + ferrocyanide
-
 ?
H2 + NAD+
-
 Cupriavidus necator H+ + NADH
-
 ?
H2 + NAD+
-
 Rhodococcus opacus H+ + NADH
-
 ?
H2 + NAD+
-
 Achromobacter denitrificans H+ + NADH
-
 ?
H2 + NAD+
-
 Achromobacter ruhlandii H+ + NADH
-
 ?
H2 + NAD+
-
 Cupriavidus necator Cd2/01 H+ + NADH
-
 ?
H2 + NAD+
-
 Achromobacter denitrificans 4a-2 H+ + NADH
-
 ?
H2 + NAD+
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + NADH
-
 ?
H2 + oxidized methyl viologen
-
 Cupriavidus necator H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Rhodococcus opacus H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Achromobacter denitrificans H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Achromobacter ruhlandii H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Cupriavidus necator Cd2/01 H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Achromobacter denitrificans 4a-2 H+ + reduced methyl viologen
-
 ?
H2 + oxidized methyl viologen
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + reduced methyl viologen
-
 ?
H2 + oxidized methylene blue
-
 Cupriavidus necator H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Rhodococcus opacus H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Achromobacter denitrificans H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Achromobacter ruhlandii H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Cupriavidus necator Cd2/01 H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Achromobacter denitrificans 4a-2 H+ + reduced methylene blue
-
 ?
H2 + oxidized methylene blue
-
 Cupriavidus necator H16 / ATCC 23440 / NCIB 10442 / S-10-1 H+ + reduced methylene blue
-
 ?

Subunits

Subunits Comment Organism
More enzyme is composed of 4 subunits Rhodococcus opacus
More enzyme is composed of 4 subunits Achromobacter denitrificans
More enzyme is composed of 4 subunits Achromobacter ruhlandii