Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Streptomyces lincolnensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tyrosine + H2O2 | Streptomyces lincolnensis | - |
L-dopa + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces lincolnensis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-affinity column chromatography and Sephadex G-25 gel filtration | Streptomyces lincolnensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-chloro-L-tyrosine + H2O2 | the enzyme performs oxidative C-H bond cleavage at C5 to generate 3-chloro-5-hydroxyl-L-tyrosine and oxygenation at C3 concomitant with a carbon-fluorine bond cleavage to yield L-dopa and chloride | Streptomyces lincolnensis | 3-chloro-5-hydroxyl-L-tyrosine + L-dopa + Cl- | - |
? | |
3-fluoro-L-tyrosine + H2O2 | the enzyme performs oxidative C-H bond cleavage at C5 to generate 3-fluoro-5-hydroxyl-L-tyrosine and oxygenation at C3 concomitant with a carbon-fluorine bond cleavage to yield L-dopa and fluoride | Streptomyces lincolnensis | 3-fluoro-5-hydroxyl-L-tyrosine + L-dopa + F- | - |
? | |
3-iodo-L-tyrosine + H2O2 | the enzyme performs oxidative C-H bond cleavage at C5 to generate 3-iodo-5-hydroxyl-L-tyrosine and oxygenation at C3 concomitant with a carbon-fluorine bond cleavage to yield L-dopa and iodide | Streptomyces lincolnensis | 3-iodo-5-hydroxyl-L-tyrosine + L-dopa + I- | - |
? | |
3-nitro-L-tyrosine + H2O2 | - |
Streptomyces lincolnensis | 3-nitro-5-hydroxyl-L-tyrosine + H2O | - |
? | |
L-tyrosine + H2O2 | - |
Streptomyces lincolnensis | L-dopa + H2O | - |
? | |
additional information | no activity with L-phenylalanine and O-methyl-L-tyrosine | Streptomyces lincolnensis | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
LmbB2 | - |
Streptomyces lincolnensis |
TyrH | - |
Streptomyces lincolnensis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Streptomyces lincolnensis |