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Literature summary for 1.11.2.1 extracted from

  • Mate, D.M.; Palomino, M.A.; Molina-Espeja, P.; Martin-Diaz, J.; Alcalde, M.
    Modification of the peroxygenative peroxidative activity ratio in the unspecific peroxygenase from Agrocybe aegerita by structure-guided evolution (2017), Protein Eng. Des. Sel., 30, 191-198 .
    View publication on PubMed
Search for more literature for 1.11.2.1

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain BJ5465 Cyclocybe aegerita

Protein Variants

Protein Variants Comment Organism
F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L the mutant enzyme exhibits enhanced activity, secretion and stability compared to the wild type enzyme Cyclocybe aegerita
S226G the mutant shows increased thermal stability compared to the wild type enzyme Cyclocybe aegerita
T120V/S226G/T320N the mutant shows reduced activity compared to the wild type enzyme Cyclocybe aegerita
T120V/S226G/T320R the mutant shows reduced activity compared to the wild type enzyme Cyclocybe aegerita

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
0.043
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
0.083
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita
0.35
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
0.49
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
0.87
-
 5-nitro-1,3-benzodioxole mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita

Organism

Organism UniProt Comment Textmining
Cyclocybe aegerita B9W4V6
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap SP column chromatography and BioSuite Q column chromatography Cyclocybe aegerita

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Cyclocybe aegerita oxidized 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O
-
 ?
5-nitro-1,3-benzodioxole + H2O2
-
 Cyclocybe aegerita 4-nitrocatechol + formic acid + H2O
-
 ?

Synonyms

Synonyms Comment Organism
UPO
-
 Cyclocybe aegerita

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
192
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
268
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
328
-
 5-nitro-1,3-benzodioxole mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita
343
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
380
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
952
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita

Cofactor

Cofactor Comment Organism Structure
heme
-
 Cyclocybe aegerita

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
393
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
406
-
 5-nitro-1,3-benzodioxole mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita
748
-
 5-nitro-1,3-benzodioxole mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
7982
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320N, at pH 4.4 and 30°C Cyclocybe aegerita
8891
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme T120V/S226G/T320R, at pH 4.4 and 30°C Cyclocybe aegerita
11424
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) mutant enzyme F12Y/A14V/R15G/A21D/V57A/L67F/V75I/I248V/F311L, at pH 4.4 and 30°C Cyclocybe aegerita