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Literature summary for 1.11.2.1 extracted from
- The coprophilous mushroom Coprinus radians secretes a haloperoxidase that catalyzes aromatic peroxygenation (2007), Appl. Environ. Microbiol., 73, 5477-5485.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.049 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians |  |
| 0.088 | - |
veratryl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 0.342 | - |
2,6-dimethoxyphenol | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians | |
| 0.584 | - |
Naphthalene | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 0.635 | - |
benzyl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 1.201 | - |
H2O2 | in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Coprinellus radians | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
enzyme fractions P II and P III, SDS-PAGE | Coprinellus radians |
| 45000 | - |
enzyme fraction P I, SDS-PAGE | Coprinellus radians |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Coprinellus radians | - |
strain DSMZ 888 | - |
Oxidation Stability
| Oxidation Stability | Organism |
|---|---|
| an H2O2 concentration of 0.7 mM is most suitable regarding enzyme activity and stability | Coprinellus radians |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | the deglycosylated protein has a molecular mass of 27000 Da, indicating a high carbohydrate content of 37% of the mature protein | Coprinellus radians |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ultrafiltration, Q Sepharose column chromatography, Mono Q column chromatography, and SEC column chromatography | Coprinellus radians |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.83 | - |
culture liquid, using veratryl alcohol as substrate, at pH 7.0 and 25°C | Coprinellus radians |
| 30.6 | - |
36.7fold purified enzyme fraction P II, using veratryl alcohol as substrate, at pH 7.0 and 25°C | Coprinellus radians |
| 31.5 | - |
37.8fold purified enzyme fraction P I, using veratryl alcohol as substrate, at pH 7.0 and 25°C | Coprinellus radians |
| 38.5 | - |
46.2fold purified enzyme fraction P III, using veratryl alcohol as substrate, at pH 7.0 and 25°C | Coprinellus radians |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) + H2O2 | - |
Coprinellus radians | ? | - |
? | |
| 2,6-dimethoxyphenol + H2O2 | - |
Coprinellus radians | ? | - |
? | |
| benzyl alcohol + H2O2 | - |
Coprinellus radians | benzaldehyde + H2O | - |
? | |
| additional information | the enzyme also brominates phenol to 2- and 4-bromophenols and selectively hydroxylates naphthalene to 1-naphthol, but shows no laccase activity | Coprinellus radians | ? | - |
? | |
| naphthalene + H2O2 | - |
Coprinellus radians | 1-naphthol + H2O | - |
? | |
| veratryl alcohol + H2O2 + H+ | - |
Coprinellus radians | veratraldehyde + H2O | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| haloperoxidase-peroxygenase | - |
Coprinellus radians |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
2,6-dimethoxyphenol | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians | |
| 15 | - |
Naphthalene | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 34 | - |
veratryl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 123 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians | |
| 176 | - |
benzyl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 471 | - |
H2O2 | in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
pH optimum for the oxidation of 2,2â-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | Coprinellus radians |
| 6 | - |
pH optimum for the oxidation of 2,6-dimethoxyphenol | Coprinellus radians |
| 7 | - |
the optimum pH of the major fraction (P II) for the oxidation of aryl alcohols is around 7.0 | Coprinellus radians |
| 7.5 | - |
pH optimum for the oxidation of veratryl alcohol | Coprinellus radians |
| 8 | - |
pH optimum for the oxidation of benzyl alcohol | Coprinellus radians |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 9 | oxidation of veratryl alcohol and benzyl alcohol occurs in a broad pH range between 2.5 and 9.0 | Coprinellus radians |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | heme-thiolate protein | Coprinellus radians | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Coprinellus radians | enzyme fraction P I shows two pI-values at 3.8 and 3.9, isoelectric focusing | - |
3.8 |
| Coprinellus radians | enzyme fraction P I shows two pI-values at 3.8 and 3.9, isoelectric focusing | - |
3.9 |
| Coprinellus radians | enzyme fraction P III, isoelectric focusing | - |
4 |
| Coprinellus radians | enzyme fraction P II, isoelectric focusing | - |
4.2 |
KCat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 5.85 | - |
2,6-dimethoxyphenol | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians | |
| 25.7 | - |
Naphthalene | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 277 | - |
benzyl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 386 | - |
veratryl alcohol | in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 392 | - |
H2O2 | in the presence of 5 mM benzyl alcohol, in sodium phosphate-citrate buffer at pH 7.0 and 25°C | Coprinellus radians | |
| 2510 | - |
2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonate) | in sodium phosphate-citrate buffer at pH 4.5 and 25°C | Coprinellus radians | |
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