BRENDA - Enzyme Database
show all sequences of 1.11.1.B7

Purification and molecular and catalytic properties of bromoperoxidase from Streptomyces phaeochromogenes

van Pee, K.H.; Lingens, F.; J. Gen. Microbiol. 131, 1911-1916 (1985)

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72500
-
2 * 72500, SDS-PAGE under denaturing and non-denaturing conditions
Streptomyces phaeochromogenes
147000
-
gel filtration
Streptomyces phaeochromogenes
Organism
Organism
UniProt
Commentary
Textmining
Streptomyces phaeochromogenes
-
RRL B-3559
-
Streptomyces phaeochromogenes RRL B-3559
-
RRL B-3559
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Streptomyces phaeochromogenes
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
675170
Streptomyces phaeochromogenes
?
-
-
-
?
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
675170
Streptomyces phaeochromogenes RRL B-3559
?
-
-
-
?
additional information
in absence or presence of bromide, the enzyme is able to catalyze the oxidation of o-dianisidine, pyrogallol and o-phenylendiamine. The enzyme ahows catalase activity
675170
Streptomyces phaeochromogenes
?
-
-
-
?
additional information
in absence or presence of bromide, the enzyme is able to catalyze the oxidation of o-dianisidine, pyrogallol and o-phenylendiamine. The enzyme ahows catalase activity
675170
Streptomyces phaeochromogenes RRL B-3559
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 72500, SDS-PAGE under denaturing and non-denaturing conditions
Streptomyces phaeochromogenes
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
bromoperoxidase activity
Streptomyces phaeochromogenes
7
-
catalase activity
Streptomyces phaeochromogenes
Cofactor
Cofactor
Commentary
Organism
Structure
heme
contains 0.91 molecules of heme per dimer, hame type IX
Streptomyces phaeochromogenes
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces phaeochromogenes
isoelectric focusing, pH-range 3.5-9.5
-
4
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
contains 0.91 molecules of heme per dimer, hame type IX
Streptomyces phaeochromogenes
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
72500
-
2 * 72500, SDS-PAGE under denaturing and non-denaturing conditions
Streptomyces phaeochromogenes
147000
-
gel filtration
Streptomyces phaeochromogenes
Purification (Commentary) (protein specific)
Commentary
Organism
-
Streptomyces phaeochromogenes
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
675170
Streptomyces phaeochromogenes
?
-
-
-
?
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
675170
Streptomyces phaeochromogenes RRL B-3559
?
-
-
-
?
additional information
in absence or presence of bromide, the enzyme is able to catalyze the oxidation of o-dianisidine, pyrogallol and o-phenylendiamine. The enzyme ahows catalase activity
675170
Streptomyces phaeochromogenes
?
-
-
-
?
additional information
in absence or presence of bromide, the enzyme is able to catalyze the oxidation of o-dianisidine, pyrogallol and o-phenylendiamine. The enzyme ahows catalase activity
675170
Streptomyces phaeochromogenes RRL B-3559
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 72500, SDS-PAGE under denaturing and non-denaturing conditions
Streptomyces phaeochromogenes
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
bromoperoxidase activity
Streptomyces phaeochromogenes
7
-
catalase activity
Streptomyces phaeochromogenes
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Streptomyces phaeochromogenes
isoelectric focusing, pH-range 3.5-9.5
-
4
Other publictions for EC 1.11.1.B7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673375
Kuusk
Purification and characterizat ...
Pseudomonas sp.
Enzyme Microb. Technol.
28
617-624
2001
-
-
-
-
-
-
2
-
-
-
2
-
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
1
-
1
-
1
1
-
1
-
-
-
-
1
-
-
-
-
2
-
-
-
-
2
-
-
-
-
1
-
-
1
-
3
1
-
-
1
-
1
-
1
1
-
-
-
-
-
-
675170
van Pee
Purification and molecular and ...
Streptomyces phaeochromogenes, Streptomyces phaeochromogenes RRL B-3559
J. Gen. Microbiol.
131
1911-1916
1985
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-
-
-
-
-
-
-
-
-
2
-
-
2
-
-
1
-
-
-
-
-
4
1
-
-
-
-
-
2
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
4
1
-
-
-
-
2
-
-
1
-
-
-
-
-
-
673605
van Pee
Detection of a bromoperoxidase ...
Streptomyces phaeochromogenes, Streptomyces phaeochromogenes NRRL B-3559
FEBS Lett.
173
5-8
1984
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-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
6
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
6
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-