BRENDA - Enzyme Database
show all sequences of 1.11.1.B7

Purification and characterization of a novel bromoperoxidase-catalase isolated from bacteria found in recycled pulp white water

Kuusk, H.; Bjorklund, M.; Rydstrom, J.; Enzyme Microb. Technol. 28, 617-624 (2001)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
azide
75 mM, 50% inhibition
Pseudomonas sp.
cyanide
0.023 mM, 50% inhibition
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77000
-
2 * 77000, SDS-PAGE
Pseudomonas sp.
153000
-
gel filtration
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
EF group 70B
-
Purification (Commentary)
Purification (Commentary)
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5283
-
-
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
673375
Pseudomonas sp.
?
-
-
-
?
Br- + H2O2 + dianisidine
-
673375
Pseudomonas sp.
?
-
-
-
?
additional information
the enzyme also shows catalase activity
673375
Pseudomonas sp.
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
2 * 77000, SDS-PAGE
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
30 min, stable
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
bromoperoxidase activity
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
4
12
30 min, stable
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
heme
presence of a protophorphyrin IX heme group
Pseudomonas sp.
pI Value
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
isoelectric focusing, pH-range 2.5-6.5
-
4.7
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
heme
presence of a protophorphyrin IX heme group
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
azide
75 mM, 50% inhibition
Pseudomonas sp.
cyanide
0.023 mM, 50% inhibition
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
77000
-
2 * 77000, SDS-PAGE
Pseudomonas sp.
153000
-
gel filtration
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5283
-
-
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione
i.e. monochlorodimedone
673375
Pseudomonas sp.
?
-
-
-
?
Br- + H2O2 + dianisidine
-
673375
Pseudomonas sp.
?
-
-
-
?
additional information
the enzyme also shows catalase activity
673375
Pseudomonas sp.
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
2 * 77000, SDS-PAGE
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
55
-
30 min, stable
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
bromoperoxidase activity
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
4
12
30 min, stable
Pseudomonas sp.
pI Value (protein specific)
Organism
Commentary
pI Value Maximum
pI Value
Pseudomonas sp.
isoelectric focusing, pH-range 2.5-6.5
-
4.7
Other publictions for EC 1.11.1.B7
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
673375
Kuusk
Purification and characterizat ...
Pseudomonas sp.
Enzyme Microb. Technol.
28
617-624
2001
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2
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2
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1
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1
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-
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1
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3
1
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-
1
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1
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1
1
-
1
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1
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2
-
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2
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1
-
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1
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3
1
-
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1
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1
-
1
1
-
-
-
-
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-
675170
van Pee
Purification and molecular and ...
Streptomyces phaeochromogenes, Streptomyces phaeochromogenes RRL B-3559
J. Gen. Microbiol.
131
1911-1916
1985
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2
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2
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1
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-
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4
1
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-
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2
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-
1
-
1
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-
1
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2
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1
-
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4
1
-
-
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-
2
-
-
1
-
-
-
-
-
-
673605
van Pee
Detection of a bromoperoxidase ...
Streptomyces phaeochromogenes, Streptomyces phaeochromogenes NRRL B-3559
FEBS Lett.
173
5-8
1984
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2
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1
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6
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1
1
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1
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6
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1
1
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