Literature summary for 1.11.1.B2 extracted from

Tanaka, N.; Wever, R.
Inhibition of vanadium chloroperoxidase from the fungus Curvularia inaequalis by hydroxylamine, hydrazine and azide and inactivation by phosphate (2004), J. Inorg. Biochem., 98, 625-631.

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Inhibitors

Inhibitors	Comment	Organism	Structure
azide	uncompetitive; uncompetitive inhibition, inhibition constant: 50 nM	Curvularia inaequalis
hydrazine	uncompetitive; uncompetitive inhibition, inhibition constant: 0.3 mM	Curvularia inaequalis
hydroxylamine	competitive and uncompetitive inhibition; inhibits rVCPO both competitively and uncompetitively. The competitive inhibition constant: 0.04 mM, uncompetitive inhibition 0.08 mM	Curvularia inaequalis
phosphate	substitution of vanadate in the active site by phosphate leads to inactivation of the enzyme. Presence of H2O2 prevents inactivation by phosphate; substitution of vanadate in the active site by phosphate leads to inactivation. Presence of H2O2 prevents the inactivation	Curvularia inaequalis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Vanadium	substitution of vanadate in the active site by phosphate leads to inactivation of the enzyme. Pervanadate is bound much more strongly to the enzyme than vanadate	Curvularia inaequalis

Organism

Organism	UniProt	Comment	Textmining
Curvularia inaequalis	P49053	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Br- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione	i.e. monochlorodimedone	Curvularia inaequalis	?	-	?
Cl- + H2O2 + 1,1-dimethyl-4-chloro-3,5-cyclohexanedione	i.e. monochlorodimedone	Curvularia inaequalis	?	-	?

Synonyms

Synonyms	Comment	Organism
vanadium chloroperoxidase	-	Curvularia inaequalis

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.04	-	hydroxylamine	competitive inhibition constant	Curvularia inaequalis
0.08	-	hydroxylamine	uncompetitive inhibition constant	Curvularia inaequalis
0.35	-	hydrazine	-	Curvularia inaequalis

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Release 2023.1 (January 2023)