Inhibitors | Comment | Organism | Structure |
---|---|---|---|
azide | no inhibition | Burkholderia pyrrocinia | |
F- | 0.18 mM, 50% inhibition | Burkholderia pyrrocinia | |
H2O2 | above 50 mM | Burkholderia pyrrocinia | |
KCN | 0.01 mM, 50% inhibition | Burkholderia pyrrocinia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
H2O2 | - |
Burkholderia pyrrocinia | |
30 | - |
Br- | - |
Burkholderia pyrrocinia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000, SDS-PAGE | Burkholderia pyrrocinia |
64000 | - |
- |
Burkholderia pyrrocinia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2 | Burkholderia pyrrocinia | enzyme is involved in the biosynthesis of the antibiotic pyrrolnitrin | aminopyrrolnitrin + ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia pyrrocinia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Burkholderia pyrrocinia |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.24 | - |
bromoperoxidase activity | Burkholderia pyrrocinia |
4.23 | - |
chloroperoxidase activity | Burkholderia pyrrocinia |
Storage Stability | Organism |
---|---|
4°C, 0.1 M sodium acetate buffer, pH 3.5-5.5, 0.1 M ammonium acetate buffer, pH 6.0-7.0, 0.1 M potassium phosphate buffer, pH 7.0-8.5, stable for over 2 months | Burkholderia pyrrocinia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2 | - |
Burkholderia pyrrocinia | aminopyrrolnitrin + ? | - |
? | |
4-(2-amino-3-chlorophenyl)pyrrole + Cl- + H2O2 | enzyme is involved in the biosynthesis of the antibiotic pyrrolnitrin | Burkholderia pyrrocinia | aminopyrrolnitrin + ? | - |
? | |
indole + Cl- + H2O2 | - |
Burkholderia pyrrocinia | oxindole + monochloroindole + H2O | - |
? | |
monochlorodimedon + Cl- + H2O2 | no activity | Burkholderia pyrrocinia | dichlorodimedon + H2O | - |
? | |
monochlorodimedone + Br- + H2O2 | - |
Burkholderia pyrrocinia | ? | - |
? | |
additional information | no peroxidase activity, no catalase activity | Burkholderia pyrrocinia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000, SDS-PAGE | Burkholderia pyrrocinia |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Burkholderia pyrrocinia |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
2 h, stable | Burkholderia pyrrocinia |
70 | - |
10 min, 50% loss of activity | Burkholderia pyrrocinia |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | 4.5 | - |
Burkholderia pyrrocinia |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3.5 | 8.5 | stable | Burkholderia pyrrocinia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | non-heme chloroperoxidase | Burkholderia pyrrocinia |