Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Drosophila melanogaster |
Protein Variants | Comment | Organism |
---|---|---|
C74A | mutation results in a decline of specific activity | Drosophila melanogaster |
N136A | mutation results in a dramatic decline of specific activity | Drosophila melanogaster |
N136D | mutation results in not detectable specific activity | Drosophila melanogaster |
N136H | mutation results in a dramatic decline of specific activity | Drosophila melanogaster |
N40A | mutation results in a decline of specific activity | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | Q9VZQ8 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA resin column chromatography and Superdex 75 gel filtration | Drosophila melanogaster |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.24 | - |
mutant enzyme N136A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates | Drosophila melanogaster |
0.4 | - |
mutant enzyme N136H, using thioredoxin and phosphatidylcholine hydroperoxide as substrates | Drosophila melanogaster |
4.29 | - |
mutant enzyme N40A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates | Drosophila melanogaster |
5.81 | - |
mutant enzyme C74A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates | Drosophila melanogaster |
14.38 | - |
wild type enzyme, using thioredoxin and phosphatidylcholine hydroperoxide as substrates | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
phosphatidylcholine hydroperoxide + thioredoxin | - |
Drosophila melanogaster | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GPX | - |
Drosophila melanogaster |