Literature summary for 1.11.1.9 extracted from

Tosatto, S.C.; Bosello, V.; Fogolari, F.; Mauri, P.; Roveri, A.; Toppo, S.; Flohe, L.; Ursini, F.; Maiorino, M.
The catalytic site of glutathione peroxidases (2008), Antioxid. Redox Signal., 10, 1515-1526.

Search for more literature for 1.11.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli JM109 cells	Drosophila melanogaster

Protein Variants

Protein Variants	Comment	Organism
C74A	mutation results in a decline of specific activity	Drosophila melanogaster
N136A	mutation results in a dramatic decline of specific activity	Drosophila melanogaster
N136D	mutation results in not detectable specific activity	Drosophila melanogaster
N136H	mutation results in a dramatic decline of specific activity	Drosophila melanogaster
N40A	mutation results in a decline of specific activity	Drosophila melanogaster

Organism

Organism	UniProt	Comment	Textmining
Drosophila melanogaster	Q9VZQ8	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA resin column chromatography and Superdex 75 gel filtration	Drosophila melanogaster

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.24	-	mutant enzyme N136A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates	Drosophila melanogaster
0.4	-	mutant enzyme N136H, using thioredoxin and phosphatidylcholine hydroperoxide as substrates	Drosophila melanogaster
4.29	-	mutant enzyme N40A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates	Drosophila melanogaster
5.81	-	mutant enzyme C74A, using thioredoxin and phosphatidylcholine hydroperoxide as substrates	Drosophila melanogaster
14.38	-	wild type enzyme, using thioredoxin and phosphatidylcholine hydroperoxide as substrates	Drosophila melanogaster

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
phosphatidylcholine hydroperoxide + thioredoxin	-	Drosophila melanogaster	?	-	?

Synonyms

Synonyms	Comment	Organism
GPX	-	Drosophila melanogaster

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Release 2023.1 (January 2023)