Cloned (Comment) | Organism |
---|---|
gene MnP13, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) as soluble protein. Coexpression with disulfide bond isomerase C (DsbC) | Gelatoporia subvermispora |
Protein Variants | Comment | Organism |
---|---|---|
additional information | four MnPs from three MnP 88 subfamilies (extralong, long, and short MnPs) in Ceriporiopsis subvermispora are selected as model proteins for developing a universal method for MnP production, overview | Gelatoporia subvermispora |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | the activity of MnP124076 toward Mn2+ is too low to obtain reliable kinetic parameters | Gelatoporia subvermispora |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | required, 2 Ca2+ per subunit | Gelatoporia subvermispora | |
Fe2+ | in the heme group | Gelatoporia subvermispora | |
Mn2+ | absolutely required for activity | Gelatoporia subvermispora |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | Gelatoporia subvermispora | - |
2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | Gelatoporia subvermispora B | - |
2 Mn(III) + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gelatoporia subvermispora | A0A2I7M8T7 | - |
- |
Gelatoporia subvermispora B | A0A2I7M8T7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant soluble His-tagged enzyme from Escherichia coli strain BL21(DE3) in presence of 1 mM CaCl2 by nickel affinity chromatography, dialysis, followed by anion exchange chromatography, ultrafiltration, and gel filtration. Heme incorporation into MnP after purification and removal of excess heme by high speed centrifugation at 50000 x g | Gelatoporia subvermispora |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora | 2 Mn(III) + 2 H2O | - |
? | |
2 Mn(II) + 2 H+ + H2O2 | - |
Gelatoporia subvermispora B | 2 Mn(III) + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | Mn2+ is absolutely required for the activity | Gelatoporia subvermispora | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? | |
2,6-dimethoxyphenol + 2 H+ + H2O2 | Mn2+ is absolutely required for the activity | Gelatoporia subvermispora B | oxidized 2,6-dimethoxyphenol + 2 H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CmMnP | - |
Gelatoporia subvermispora |
MnP124076 | - |
Gelatoporia subvermispora |
MnP13 | gene name, UniProt | Gelatoporia subvermispora |
More | cf. EC 1.11.1.13 | Gelatoporia subvermispora |
short PMnP | - |
Gelatoporia subvermispora |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | assay at | Gelatoporia subvermispora |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
substrate 2,6-dimethoxyphenol | Gelatoporia subvermispora |
5 | - |
substrate Mn(II) | Gelatoporia subvermispora |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme b | - |
Gelatoporia subvermispora |
General Information | Comment | Organism |
---|---|---|
evolution | MnP124076 is the only short MnP found in Ceriporiopsis subvermispora, MnP157986 is an extralong MnP and has the longest amino acid sequence. MnP50297 and MnP117436 are an extralong MnP and a long MnP, respectively, and they are the most abundantly produced MnPs in each subfamily by Ceriporiopsis subvermispora grown in aspen wood-containing medium | Gelatoporia subvermispora |