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Literature summary for 1.11.1.7 extracted from

  • Askari, H.; Rahimiana, A.; Aminiana, M.
    Purification and biochemical characterization of two anionic peroxidase isoenzymes from Raphanus sativus L. var niger roots (2022), Appl. Biochem. Biotechnol., 194, 2219-2235.
    View publication on PubMed
Search for more literature for 1.11.1.7

Activating Compound

Activating Compound Comment Organism Structure
Mg2+ 5 mM, 123% of initial activity for isoform PRB-A. 112% of inital activity for isoform PRB-B Raphanus sativus var. niger
Mn2+ 5 mM, 143% of initial activity for isoform PRB-A. No activation of isoform PRB-B Raphanus sativus var. niger
Ni2+ 5 mM, 139% of initial activity for isoform PRB-A. No activation of isoform PRB-B Raphanus sativus var. niger
Zn2+ 5 mM, 143% of initial activity for isoform PRB-A. 121% of inital activity for isoform PRB-B Raphanus sativus var. niger

Inhibitors

Inhibitors Comment Organism Structure
azide
-
 Raphanus sativus var. niger
citrate
-
 Raphanus sativus var. niger
EDTA
-
 Raphanus sativus var. niger
guanidine hydrochloride less than 10% activity at 1M guanidine hydrochloride Raphanus sativus var. niger
SDS about 75% loss of activity at 2% W/V SDS Raphanus sativus var. niger
Urea loss of more than 50% of its activity at 1M urea Raphanus sativus var. niger

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.043
-
 H2O2 isoform PRB-A, pH 7, pH 7, 23°C Raphanus sativus var. niger
0.067
-
 H2O2 isoform PRB-B, pH 7, pH 7, 23°C Raphanus sativus var. niger
10.63
-
 4-aminoantipyrine isoform PRB-A, pH 7, pH 7, 23°C Raphanus sativus var. niger
15.38
-
 4-aminoantipyrine isoform PRB-B, pH 7, pH 7, 23°C Raphanus sativus var. niger

Organism

Organism UniProt Comment Textmining
Raphanus sativus var. niger
-
-
-

Purification (Commentary)

Purification (Comment) Organism
protein extraction by a combination of freeze-thawing and grinding, ultrafiltration and diafiltration, ammonium sulfate precipitation, DEAE anion-exchange chromatography, and concanavalin A affinity chromatography Raphanus sativus var. niger

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Raphanus sativus var. niger
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
166.7
-
 pH 7, 23°C, assay mixture contains 18 mM 4-aminoantipyrine, 0.2 mM H2O2, and 85 mM phenol Raphanus sativus var. niger

Storage Stability

Storage Stability Organism
37°C, stable for at least 120 days Raphanus sativus var. niger
4°C, stable for at least 120 days Raphanus sativus var. niger

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-aminoantipyrine + phenol + H2O2
-
 Raphanus sativus var. niger quinoneimine + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 110000, isoform BRP-A, 1 * 97000, isoform BRP-B, SDS-PAGE Raphanus sativus var. niger

Synonyms

Synonyms Comment Organism
black radish peroxidase A
-
 Raphanus sativus var. niger
black radish peroxidase B
-
 Raphanus sativus var. niger
BRP-A
-
 Raphanus sativus var. niger
BRP-B
-
 Raphanus sativus var. niger

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 both isoforms BRP-A and BRP-B Raphanus sativus var. niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 isoform BRP-A Raphanus sativus var. niger
6
-
 isoform BRP-B Raphanus sativus var. niger