Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.7 extracted from

  • Krainer, F.; Pletzenauer, R.; Rossetti, L.; Herwig, C.; Glieder, A.; Spadiut, O.
    Purification and basic biochemical characterization of 19 recombinant plant peroxidase isoenzymes produced in Pichia pastoris (2014), Protein Expr. Purif., 95, 104-112 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.7

Application

Application Comment Organism
synthesis expression of 19 individual HRP isoenzymes recombinantly in the yeast Pichia pastoris and optimization of a 2-step purification strategy Armoracia rusticana

Cloned(Commentary)

Cloned (Comment) Organism
expression in Pichia pastoris Armoracia rusticana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.01
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 37°C Armoracia rusticana
1.95
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 37°C Armoracia rusticana
3.5
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 6.5, 37°C Armoracia rusticana

Organism

Organism UniProt Comment Textmining
Armoracia rusticana K7ZW28
-
-
Armoracia rusticana K7ZW57
-
-
Armoracia rusticana K7ZWW6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Armoracia rusticana ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
HRP_A2A
-
 Armoracia rusticana
HRP_C1A
-
 Armoracia rusticana
HRP_E5
-
 Armoracia rusticana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 stable for 60 min Armoracia rusticana
60
-
 half-life 64 min Armoracia rusticana

pI Value

Organism Comment pI Value Maximum pI Value
Armoracia rusticana calculated
-
 4.8
Armoracia rusticana calculated
-
 5.4
Armoracia rusticana calculated
-
 9