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Literature summary for 1.11.1.7 extracted from
- Purification and characterization of peroxidase from sprouted green gram (Vigna radiata) roots and removal of phenol and p-chlorophenol by immobilized peroxidase (2017), J. Sci. Food Agric., 97, 3249-3260 .
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 5 mM, 56% residual activity | Vigna radiata |  |
| Ca2+ | 5 mM, 47% residual activity | Vigna radiata | |
| dithiothreitol | 5 mM, complete loss of activity | Vigna radiata | |
| Hg2+ | 5 mM, 17% residual activity | Vigna radiata | |
| hydrazine | 5 mM, 45% residual activity | Vigna radiata | |
| K+ | 5 mM, 41% residual activity | Vigna radiata | |
| L-cysteine | 10 mM, complete loss of activity | Vigna radiata | |
| Mg2+ | 5 mM, 46% residual activity | Vigna radiata | |
| Na+ | 5 mM, 53% residual activity | Vigna radiata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.045 | - |
H2O2 | pH 5.5, temperature not specified in the publication | Vigna radiata | |
| 1.28 | - |
o-Dianisidine | pH 5.5, temperature not specified in the publication | Vigna radiata | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 3fold activation | Vigna radiata | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Vigna radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| from root | Vigna radiata |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| root | - |
Vigna radiata | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85.4 | - |
pH 5.5, temperature not specified in the publication | Vigna radiata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| benzidine + H2O2 | 26% of the activity with tetramethylbenzidine | Vigna radiata | ? + H2O | - |
? | |
| diaminobenzidine + H2O2 | 16% of the activity with tetramethylbenzidine | Vigna radiata | ? + H2O | - |
? | |
| o-dianisidine + H2O2 | 66% of the activity with tetramethylbenzidine | Vigna radiata | ? + H2O | - |
? | |
| p-phenylenediamine + H2O2 | 14% of the activity with tetramethylbenzidine | Vigna radiata | ? + H2O | - |
? | |
| tetramethylbenzidine + H2O2 | - |
Vigna radiata | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE | Vigna radiata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Vigna radiata |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | - |
Vigna radiata |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Vigna radiata | peroxidase activity increases by 300fold in 5-day germinated seed | up |
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