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Literature summary for 1.11.1.7 extracted from

  • Wen, B.; Baker, M.R.; Zhao, H.; Cui, Z.; Li, Q.X.
    Expression and characterization of windmill palm tree (Trachycarpus fortunei) peroxidase by Pichia pastoris (2017), J. Agric. Food Chem., 65, 4676-4682 .
    View publication on PubMed
Search for more literature for 1.11.1.7

Cloned(Commentary)

Cloned (Comment) Organism
expression of codon-optimized gene in Pichia pastoris Trachycarpus fortunei

Inhibitors

Inhibitors Comment Organism Structure
Fe2+ 5 mM, 13% residual activity Trachycarpus fortunei
Mn2+ 5 mM, 10% residual activity Trachycarpus fortunei
additional information presence of Mg2+, Cu2+, Fe3+, Ni2+, citric acid, and urea has no effect on the peroxidase activity Trachycarpus fortunei

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.16
-
 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) pH 3.0, 23°C Trachycarpus fortunei
0.46
-
 H2O2 pH 3.0, 23°C Trachycarpus fortunei

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 10%, 81% residual activity Trachycarpus fortunei

Organism

Organism UniProt Comment Textmining
Trachycarpus fortunei A0A0A0Y4H8
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein recombinant protein, about 23% glycan content Trachycarpus fortunei

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Trachycarpus fortunei
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
112
-
 pH 3.0, 23°C Trachycarpus fortunei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?
catechol + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?
guaiacol + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?
additional information no substrate: ferulic acid Trachycarpus fortunei ?
-
 ?
o-dianisidine + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?
o-phenylenediamine + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?
pyrogallol + H2O2
-
 Trachycarpus fortunei ? + H2O
-
 ?

Subunits

Subunits Comment Organism
? x * 43000, SDS-PAGE, x * 32200, calculated for unglycosylated form Trachycarpus fortunei

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
 24 h, 93% residual activity Trachycarpus fortunei
80
-
 24 h, 40% residual activity Trachycarpus fortunei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3
-
-
 Trachycarpus fortunei

pH Range

pH Minimum pH Maximum Comment Organism
2 4 more than 93% of maximum activity within Trachycarpus fortunei

pH Stability

pH Stability pH Stability Maximum Comment Organism
1 2 24 h, 75% residual activity Trachycarpus fortunei
2
-
 24 h, no loss of activity Trachycarpus fortunei