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Literature summary for 1.11.1.7 extracted from
- Characterization and application of a novel class II thermophilic peroxidase from Myceliophthora thermophila in biosynthesis of polycatechol (2015), Enzyme Microb. Technol., 75-76, 49-56 .
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | heterologous production in Pichia pastoris is greatlyenhanced by the addition of hemin with a titer of 0.41 U/ml peroxidase activity at the second day ofincubation | Thermothelomyces thermophilus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermothelomyces thermophilus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| H2O2 | enzyme retains more than 80% of its initial activity after 24 h incubation in 5000fold molar excess of H2O2 | Thermothelomyces thermophilus |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermothelomyces thermophilus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermothelomyces thermophilus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.49 | - |
substrate guaiacol, pH 5, 60°C | Thermothelomyces thermophilus |
| 0.55 | - |
substrate 2,6-dimethyloxyphenol, pH 5, 60°C | Thermothelomyces thermophilus |
| 0.63 | - |
substrate catechol, pH 5, 60°C | Thermothelomyces thermophilus |
| 2.46 | - |
substrate pyrogallol, pH 5, 60°C | Thermothelomyces thermophilus |
| 4.13 | - |
substrate hydroquinone, pH 5, 60°C | Thermothelomyces thermophilus |
| 178 | - |
substrate 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid), pH 5, 60°C | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2 guaiacol + H2O2 | - |
Thermothelomyces thermophilus | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
| 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Thermothelomyces thermophilus | ? | - |
? | |
| 2,6-dimethyloxyphenol + H2O2 | - |
Thermothelomyces thermophilus | ? + H2O | - |
? | |
| catechol + H2O2 | - |
Thermothelomyces thermophilus | ? + H2O | - |
? | |
| hydroquinone + H2O2 | - |
Thermothelomyces thermophilus | ? + H2O | - |
? | |
| additional information | no substrates: veratryl alcohol, reactive black 5, Mn2+ | Thermothelomyces thermophilus | ? | - |
? | |
| pyrogallol + H2O2 | - |
Thermothelomyces thermophilus | ? + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, SDS-PAGE, x * 38500, calculated, x * 39000, SDS-PAGE of deglycosylated protein | Thermothelomyces thermophilus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| PerII | - |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
stable up to | Thermothelomyces thermophilus |
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