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Literature summary for 1.11.1.7 extracted from

  • Chaplin, A.K.; Wilson, M.T.; Worrall, J.A.R.
    Kinetic characterisation of a dye decolourising peroxidase from Streptomyces lividans new insight into the mechanism of anthraquinone dye decolourisation (2017), Dalton Trans., 46, 9420-9429 .
    View publication on PubMed
Search for more literature for 1.11.1.7

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.73
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 7.0, 25°C Streptomyces lividans
3.1
-
 K4[Fe(CN)6] pH 5.0, 25°C Streptomyces lividans
5.6
-
 2,6-dimethyloxyphenol pH 9.0, 25°C Streptomyces lividans
10.4
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 5.0, 25°C Streptomyces lividans
15.3
-
 2,6-dimethyloxyphenol pH 7.0, 25°C Streptomyces lividans
16
-
 veratryl alcohol pH 5.0, 25°C Streptomyces lividans
16.3
-
 K4[Fe(CN)6] pH 7.0, 25°C Streptomyces lividans
17.5
-
 guaiacol pH 7.0, 25°C Streptomyces lividans
28.1
-
 guaiacol pH 10.5, 25°C Streptomyces lividans

Organism

Organism UniProt Comment Textmining
Streptomyces lividans
-
-
-
Streptomyces lividans SLI4211
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 guaiacol + H2O2
-
 Streptomyces lividans 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
2 guaiacol + H2O2
-
 Streptomyces lividans SLI4211 3,3'-dimethoxy-4,4'-biphenylquinone + H2O
-
 ?
2 K4[Fe(CN)6] + H2O2
-
 Streptomyces lividans K3[Fe(CN)6] + H2O + K2O
-
 ?
2 K4[Fe(CN)6] + H2O2
-
 Streptomyces lividans SLI4211 K3[Fe(CN)6] + H2O + K2O
-
 ?
2 veratryl alcohol + H2O2
-
 Streptomyces lividans 2 veratraldehyde + H2O
-
 ?
2 veratryl alcohol + H2O2
-
 Streptomyces lividans SLI4211 2 veratraldehyde + H2O
-
 ?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Streptomyces lividans ?
-
 ?
2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) + H2O2
-
 Streptomyces lividans SLI4211 ?
-
 ?
2,6-dimethyloxyphenol + H2O2
-
 Streptomyces lividans ? + H2O
-
 ?
additional information for most substrates, the value of kcat/Km measured by steady-state kinetics is equal to the slowest step in catalysis measured by stopped-flow spectroscopy, namely the decay of the ferryl FeIV=O species (compound II) to form the ferric species Streptomyces lividans ?
-
 ?
additional information for most substrates, the value of kcat/Km measured by steady-state kinetics is equal to the slowest step in catalysis measured by stopped-flow spectroscopy, namely the decay of the ferryl FeIV=O species (compound II) to form the ferric species Streptomyces lividans SLI4211 ?
-
 ?
reactive blue 19 + H2O2
-
 Streptomyces lividans ? + H2O
-
 ?

Synonyms

Synonyms Comment Organism
DtpA
-
 Streptomyces lividans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.054
-
 guaiacol pH 7.0, 25°C Streptomyces lividans
0.1
-
 2,6-dimethyloxyphenol pH 7.0, 25°C Streptomyces lividans
0.32
-
 2,6-dimethyloxyphenol pH 9.0, 25°C Streptomyces lividans
0.4
-
 guaiacol pH 10.5, 25°C Streptomyces lividans
0.5
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 5.0, 25°C Streptomyces lividans
0.82
-
 veratryl alcohol pH 5.0, 25°C Streptomyces lividans
1.12
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 7.0, 25°C Streptomyces lividans
109
-
 K4[Fe(CN)6] pH 7.0, 25°C Streptomyces lividans
295
-
 K4[Fe(CN)6] pH 5.0, 25°C Streptomyces lividans

Cofactor

Cofactor Comment Organism Structure
heme spectra are typical of high valent heme iron intermediate states generated in the catalytic cycle of a peroxidase Streptomyces lividans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0031
-
 guaiacol pH 7.0, 25°C Streptomyces lividans
0.0065
-
 2,6-dimethyloxyphenol pH 7.0, 25°C Streptomyces lividans
0.014
-
 guaiacol pH 10.5, 25°C Streptomyces lividans
0.048
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 5.0, 25°C Streptomyces lividans
0.051
-
 veratryl alcohol pH 5.0, 25°C Streptomyces lividans
0.057
-
 2,6-dimethyloxyphenol pH 9.0, 25°C Streptomyces lividans
1.53
-
 2,2'-azino-bis(3-ethylbenzthiazole-6-sulfonic acid) pH 7.0, 25°C Streptomyces lividans
6.7
-
 K4[Fe(CN)6] pH 7.0, 25°C Streptomyces lividans
95
-
 K4[Fe(CN)6] pH 5.0, 25°C Streptomyces lividans