Protein Variants | Comment | Organism |
---|---|---|
F130A/F142A/ F143A/F179A | isoform HRP C, mutant shows marginal improvement in stability | Armoracia rusticana |
F142A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
F143A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
F179A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
F68A | isoform HRP C, mutation in a residue vulnerable to modification by phenoxyl radicals, mutant still exhibits rapid radical inactivation | Armoracia rusticana |
F68A/F142A/F143A/F179A | isoform HRP C, dramatic enhancement of radical stability by retaining 41% of its initial activity in conditions when wild-type is completely inactivated | Armoracia rusticana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
phenoxy radical | phenylalanine residues are vulnerable to modification by phenoxyl radicals. Radical coupling does not change the secondary structure or the active site of HRP isoform C | Armoracia rusticana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.966 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
0.966 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.06 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.72 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.82 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
2.04 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
2.16 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
2.69 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Armoracia rusticana | P80679 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
commercial preparation | - |
Armoracia rusticana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 guaiacol + H2O2 | - |
Armoracia rusticana | 3,3'-dimethoxy-4,4'-biphenylquinone + H2O | - |
? | |
2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? | |
phenol + H2O2 | - |
Armoracia rusticana | ? + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
HRP A2 | - |
Armoracia rusticana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.952 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.24 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.41 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.44 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.55 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.83 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
2.09 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
2.3 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.61 | - |
guaiacol | mutant F142A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
0.86 | - |
guaiacol | mutant F68A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
0.89 | - |
guaiacol | mutant F143A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
0.89 | - |
guaiacol | mutant F68A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.01 | - |
guaiacol | mutant F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.07 | - |
guaiacol | mutant F130A/F142A/ F143A/F179A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.46 | - |
guaiacol | mutant F130A, pH 5, temperature not specified in the publication | Armoracia rusticana | |
1.49 | - |
guaiacol | wild-type isoform C, pH 5, temperature not specified in the publication | Armoracia rusticana |