Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Periodate | completely and competitively inhibited by periodate | Beta vulgaris | |
Sodium azide | non-competitive inhibitor | Beta vulgaris |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.113 | - |
H2O2 | at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) | Beta vulgaris | |
2.134 | - |
o-Dianisidine | at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) | Beta vulgaris | |
5.05 | - |
fructosyl-6-phosphate alpha-Boc-L-lysine | at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) | Beta vulgaris | |
98.61 | - |
guaiacol | at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) | Beta vulgaris |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Beta vulgaris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Beta vulgaris | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate fractionation and DEAE cellulose column chromatography | Beta vulgaris |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root hair | - |
Beta vulgaris | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the enzyme from crude extract has a specific activity of 700 units the purified enzyme has a specific activity of 10500 units, one unit of enzyme activity refers to the rate of change of 1 OD per min per mg protein | Beta vulgaris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 | - |
Beta vulgaris | ? | - |
? | |
guaiacol + H2O2 | - |
Beta vulgaris | tetraguaiacol + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Beta vulgaris | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
POD | - |
Beta vulgaris |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
retains over 70% of the activity for 20 min at 70°C | Beta vulgaris |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Beta vulgaris |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | stable over a wide range at pH 3-9 with highest stability between pH 6 and 8 | Beta vulgaris |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
Periodate | at 25°C in 0.2 M sodium phosphate buffer (pH 6.0) | Beta vulgaris |