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Literature summary for 1.11.1.7 extracted from
- Purification, cloning and characterization of a novel peroxidase isozyme from sweetpotatoes (Ipomoea batatas) (2007), Biochim. Biophys. Acta, 1774, 1422-1430.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
SDS-PAGE | Ipomoea batatas |
| 42029 | - |
1 * 42029, MALDI mass spectrometry | Ipomoea batatas |
| 42030 | - |
MALDI mass spectrometry | Ipomoea batatas |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ipomoea batatas | Q9LEH3 | sweet potato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| MonoQ column chromatography, DEAE-FF Sepharose column chromatography, and concanavalin A column chromatography | Ipomoea batatas |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, in 0.1 M phosphate buffer at pH 5.5, several months, without loss of activity | Ipomoea batatas |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis-(3-ethyl-6-benzothiazolinsulfonate) + H2O2 | 100% activity | Ipomoea batatas | ? | - |
? |  |
| guaiacol + H2O2 | 74% activity compared to 2,2'-azino-bis-(3-ethyl-6-benzothiazolinsulfonate) | Ipomoea batatas | tetraguaiacol + H2O | - |
? | |
| iodide + H2O2 | 15% activity compared to 2,2'-azino-bis-(3-ethyl-6-benzothiazolinsulfonate) | Ipomoea batatas | ? | - |
? | |
| o-dianisidine + H2O2 | 90% activity compared to 2,2'-azino-bis-(3-ethyl-6-benzothiazolinsulfonate) | Ipomoea batatas | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42029, MALDI mass spectrometry | Ipomoea batatas |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Prx15 | - |
Ipomoea batatas |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Ipomoea batatas |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 7 | - |
Ipomoea batatas |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Ipomoea batatas | isoelectric focusing | - |
4.9 |
| Ipomoea batatas | theoretical value | - |
5.07 |
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