Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 4% inhibition at 5 mM, at 37°C | Citrus jambhiri | |
Hg2+ | 80% inhibition at 5 mM, at 37°C | Citrus jambhiri | |
Li+ | 22% inhibition at 5 mM, at 37°C | Citrus jambhiri | |
Ni2+ | 10% inhibition at 5 mM, at 37°C | Citrus jambhiri | |
Zn2+ | 13% inhibition at 5 mM, at 37°C | Citrus jambhiri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.54 | - |
H2O2 | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri | |
2 | 3 | pyrogallol | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri | |
2.85 | - |
o-phenylenediamine | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri | |
5 | - |
guaiacol | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri | |
11 | - |
o-Dianisidine | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri | |
125 | - |
catechol | in 50 mM sodium acetate buffer, at pH 5.5 | Citrus jambhiri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 8% increased activity at 5 mM, at 37°C | Citrus jambhiri | |
additional information | not stimulated by Co2+, Ca2+, Mn2+, and Fe2+ | Citrus jambhiri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
SDS-PAGE | Citrus jambhiri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Citrus jambhiri | - |
cultivar Adalia | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose chromatography, carboxymethyl-cellulose column chromatography, and Sephacryl S-200 gel filtration | Citrus jambhiri |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
exocarp | - |
Citrus jambhiri | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
the crude extract has a specific activity of 751 units/mg protein, the 37.6fold purified enzyme has a specific activity of 28261 units/mg protein 1 unit of peroxidase activity is defined as the amount of enzyme that increases the optical density 1.0 per min | Citrus jambhiri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
catechol + H2O2 | 4.0% activity compared to guaiacol | Citrus jambhiri | ? | - |
? | |
guaiacol + H2O2 | 100% activity | Citrus jambhiri | tetraguaiacol + H2O | - |
? | |
additional information | not active towards L-tyrosine, ascorbic acid, alpha-naphthylamine, p-aminoantipyrine, potassium ferrocyanide, potassium iodide, and NADH | Citrus jambhiri | ? | - |
? | |
o-dianisidine + H2O2 | 93.7% activity compared to guaiacol | Citrus jambhiri | ? | - |
? | |
o-phenylenediamine + H2O2 | 171.7% activity compared to guaiacol | Citrus jambhiri | ? | - |
? | |
pyrogallol + H2O2 | 6.2% activity compared to guaiacol | Citrus jambhiri | purpurogallin + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cationic peroxidase | - |
Citrus jambhiri |
POII | - |
Citrus jambhiri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Citrus jambhiri |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 70 | stable at 10-40°C and unstable above 50°C, 70-80% of POII activity is lost at 50, 60, and 70°C after 15, 10, and 5 min of incubation, respectively | Citrus jambhiri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
- |
Citrus jambhiri |