General Stability | Organism |
---|---|
a 10% and 74% decrease in activity of native and deglycosylated peroxidase, respectively, is observed after 3 h incubation with trypsin | Brassica napus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.025 | - |
H2O2 | 25°C, pH 6.0, partially deglycosylated enzyme | Brassica napus | |
0.055 | - |
H2O2 | 25°C, pH 6.0, native enzyme | Brassica napus | |
0.9 | - |
2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) | 25°C, pH 6.0, partially deglycosylated enzyme | Brassica napus | |
1.1 | - |
2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) | 25°C, pH 6.0, native enzyme | Brassica napus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Brassica napus | - |
turnip | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 9.1% carbohydrate content, intact oligosaccacharide chains are required for full peroxidase activity | Brassica napus |
Purification (Comment) | Organism |
---|---|
cation exchange, hydrophobic interaction chromatography | Brassica napus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Brassica napus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | - |
Brassica napus | oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
approx. 50% loss of native and deglycosylated peroxidase activity after 3 min and 1 min, respectively | Brassica napus |