Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nicotiana tabacum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2.5fold and 1.5fold decrease in the second order rate constant for H2O2 and ABTS, respectively, in the presence of 50 mM Ca2+ | Nicotiana tabacum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | glycosylation has no effect on the kinetic properties and Ca2+ inhibition | Nicotiana tabacum |
Purification (Comment) | Organism |
---|---|
recombinant enzyme, 6 M urea, reactivation, ultrafiltration, gel filtration | Nicotiana tabacum |
Renatured (Comment) | Organism |
---|---|
refolding of recombinant enzyme after urea denaturation | Nicotiana tabacum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1100 | - |
recombinant enzyme | Nicotiana tabacum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
reduced 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O2 | trivial name ABTS, ping-pong mechanism | Nicotiana tabacum | oxidized 2,2'-azino-bis-(3-ethylbenzthiazole-6-sulfonic acid) + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TOP | - |
Nicotiana tabacum |