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Literature summary for 1.11.1.7 extracted from

  • Shin, K.S.; Oh, I.K.; Kim, C.J.
    Production and purification of Remazol Brilliant Blue R decolorizing peroxidase from the culture filtrate of Pleurotus ostreatus (1997), Appl. Environ. Microbiol., 63, 1744-1748.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.7

Inhibitors

Inhibitors Comment Organism Structure
KCN 76% inhibition at 0.1 mM Pleurotus ostreatus
L-cysteine 94% inhibition at 0.1 mM Pleurotus ostreatus
Na2S2O5 100% inhibition at 0.1 mM Pleurotus ostreatus
NaN3 95% inhibition at 0.1 mM Pleurotus ostreatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.006
-
 Crystal violet
-
 Pleurotus ostreatus
0.011
-
 Remazol Brilliant Blue R
-
 Pleurotus ostreatus
0.017
-
 Phenol red
-
 Pleurotus ostreatus
0.021
-
 sinapinic acid
-
 Pleurotus ostreatus
0.028
-
 syringic acid
-
 Pleurotus ostreatus
0.029
-
 malachite green
-
 Pleurotus ostreatus
0.033
-
 H2O2
-
 Pleurotus ostreatus
0.054
-
 vanillic acid
-
 Pleurotus ostreatus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pleurotus ostreatus
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
71000
-
 1 * 71000, SDS-PAGE Pleurotus ostreatus
73000
-
 gel filtration Pleurotus ostreatus

Organism

Organism UniProt Comment Textmining
Pleurotus ostreatus
-
-
-
Pleurotus ostreatus NFFA
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 38% carbohydrate by weight Pleurotus ostreatus

Purification (Commentary)

Purification (Comment) Organism
-
 Pleurotus ostreatus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6
-
 with Remazol Brilliant Blue R Pleurotus ostreatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
crystal violet + H2O2
-
 Pleurotus ostreatus ?
-
 ?
crystal violet + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
malachite green + H2O2
-
 Pleurotus ostreatus ?
-
 ?
phenol red + H2O2
-
 Pleurotus ostreatus ?
-
 ?
phenol red + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
Remazol Brilliant Blue R + H2O2
-
 Pleurotus ostreatus ?
-
 ?
Remazol Brilliant Blue R + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
sinapic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?
sinapic acid + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
syringic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?
syringic acid + H2O2
-
 Pleurotus ostreatus NFFA ?
-
 ?
vanillic acid + H2O2
-
 Pleurotus ostreatus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 71000, SDS-PAGE Pleurotus ostreatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
30
-
 stable at pH 4-6 for 1 h Pleurotus ostreatus
70
-
 complete inactivation Pleurotus ostreatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 3.5 at 25°C Pleurotus ostreatus

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 6 stable at 30°C for 1 h Pleurotus ostreatus