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Literature summary for 1.11.1.6 extracted from

  • Karakus, Y.; Goc, G.; Balci, S.; Yorke, B.; Trinh, C.; McPherson, M.; Pearson, A.
    Identification of the site of oxidase substrate binding in Scytalidium thermophilum catalase (2018), Acta Crystallogr. Sect. D, 74, 979-985 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.11.1.6

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapor diffusion method. Crystal structure of the complex of the catalase with the classical catalase inhibitor 3-amino-1,2,4-triazole is determined at 1.95 A resolution Mycothermus thermophilus

Protein Variants

Protein Variants Comment Organism
E316F kcat/Km is 2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
E316H kcat/Km is 2.6fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
H246W kcat/Km is 5.3fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
I313F slight increase in kcat/Km as compared to kcat/Km of wild-type enzyme Mycothermus thermophilus
I314F kcat/Km is 5.2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
L321A kcat/Km is 1.2fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
V536A kcat/Km is 1.6fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus
V536W kcat/Km is 5.1fold lower than kcat/Km for wild-type enzyme Mycothermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
10
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
11
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
20
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
33
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
40
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
50
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
67
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
600
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 H2O2 Mycothermus thermophilus
-
 O2 + 2 H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Mycothermus thermophilus M4GGR7
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.033
-
 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
0.085
-
 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus
0.092
-
 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
0.107
-
 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
0.163
-
 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
0.173
-
 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
0.213
-
 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
0.232
-
 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
0.236
-
 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
 Mycothermus thermophilus O2 + 2 H2O
-
 ?

Subunits

Subunits Comment Organism
homotetramer
-
 Mycothermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
152000
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
194000
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
196000
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
203410
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
205000
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
261000
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
274000
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus
860500
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
2402000
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus

Cofactor

Cofactor Comment Organism Structure
heme the homotetramer contains a heme d in each active site Mycothermus thermophilus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3800
-
 H2O2 pH 7.0, 60°C, mutant enzyme H246W Mycothermus thermophilus
3900
-
 H2O2 pH 7.0, 60°C, mutant enzyme I314F Mycothermus thermophilus
4000
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536W Mycothermus thermophilus
7900
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316H Mycothermus thermophilus
9800
-
 H2O2 pH 7.0, 60°C, mutant enzyme E316F Mycothermus thermophilus
12900
-
 H2O2 pH 7.0, 60°C, mutant enzyme V536A Mycothermus thermophilus
20300
-
 H2O2 pH 7.0, 60°C, wild-type enzyme Mycothermus thermophilus
20500
-
 H2O2 pH 7.0, 60°C, mutant enzyme I313F Mycothermus thermophilus
24300
-
 H2O2 pH 7.0, 60°C, mutant enzyme L321A Mycothermus thermophilus