Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.11.1.6 extracted from

  • Fu, X.; Wang, W.; Hao, J.; Zhu, X.; Sun, M.
    Purification and characterization of catalase from marine bacterium Acinetobacter sp. YS0810 (2014), BioMed Res. Int., 2014, 409626 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

Inhibitors Comment Organism Structure
3-Amino-1,2,4-triazole 1 mM, 86% inhibition Acinetobacter sp. YS0810
Hydroxylamine hydrochloride 0.05 mM, 78% inhibition Acinetobacter sp. YS0810
Sodium azide 0.05 mM, 69% inhibition Acinetobacter sp. YS0810

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
229000
-
gel filtration Acinetobacter sp. YS0810

Organism

Organism UniProt Comment Textmining
Acinetobacter sp. YS0810 A0A023UHK6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Acinetobacter sp. YS0810

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37318
-
pH 7.0, 25°C Acinetobacter sp. YS0810

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 H2O2
-
Acinetobacter sp. YS0810 O2 + 2 H2O
-
?
additional information no substrate: o-phenylenediamine. The purified catalase is not reduced by sodium dithionite Acinetobacter sp. YS0810 ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57256, mass spectrometry, 4 * 57000, SDS-PAGE Acinetobacter sp. YS0810

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Acinetobacter sp. YS0810

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 more than 80% of maximum activity Acinetobacter sp. YS0810

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
78% residual activity at pH 7.0 under 60?C for 30 min Acinetobacter sp. YS0810
70
-
30 min, almost complete inactivation Acinetobacter sp. YS0810

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
11
-
-
Acinetobacter sp. YS0810

pH Range

pH Minimum pH Maximum Comment Organism
8 12 raspid decrease in activity below and above Acinetobacter sp. YS0810

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 12
-
Acinetobacter sp. YS0810
10
-
4°C for 3 h, 95% residual activity Acinetobacter sp. YS0810
11
-
4°C for 3 h, 78% residual activity Acinetobacter sp. YS0810

Cofactor

Cofactor Comment Organism Structure
heme protoporphyrin IX, protein displays a Soret peak at 405 nm Acinetobacter sp. YS0810