Application | Comment | Organism |
---|---|---|
industry | hydrogen peroxide scavenging enzyme | Serratia marcescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78 | - |
H2O2 | in 50 mM NaH2PO4-Na2HPO4 buffer (pH 7.0) at 20°C | Serratia marcescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
4 * 58000, SDS-PAGE | Serratia marcescens |
230000 | - |
gel filtration | Serratia marcescens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Serratia marcescens | D7RJ66 | - |
- |
Serratia marcescens SYBC08 | D7RJ66 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE column chromatography, and Sephacryl Tm S-200 gel filtration | Serratia marcescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
14490 | - |
crude extract, pH 7.0, at 20°C | Serratia marcescens |
199600 | - |
after 13.8fold purification, pH 7.0, at 20°C | Serratia marcescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Serratia marcescens | O2 + H2O | - |
? | |
H2O2 | - |
Serratia marcescens SYBC08 | O2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 58000, SDS-PAGE | Serratia marcescens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
- |
Serratia marcescens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
- |
the enzyme has 78% activity at 0°C | Serratia marcescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | 70 | at 60°C, the enzyme is stable for 240 min, at 65°C the enzyme retains more than 55% of its activity by incubating the enzyme at pH 7.0 after 225 min, at 70°C, the enzyme can retain 57% of its initial activity after incubating at pH 7.0 for 90 min | Serratia marcescens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Serratia marcescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 11 | - |
Serratia marcescens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Serratia marcescens |
General Information | Comment | Organism |
---|---|---|
physiological function | catalase plays a significant role in preventing Serratia marcescens against cellular damage through hydrogen peroxide | Serratia marcescens |